A1H4
93062220, Reactivity: Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93062220-DNA-5UG
pkg of vial of cells 93062220-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin)
technique(s)
cell culture | mammalian: suitable
isotype
IgG1, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
Madin-Darby canine kidney (MDCK) cells
Application
Study of epithelial occluding barriers
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
The antibody is directed against a 188kDa polypeptide present on the basolateral plasma membrane of Madin-Darby canine kidney (MDCK) cells. This polypeptide is the same as, or closely related to, the cell adhesion molecule uvomorulin.
Preparation Note
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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