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Merck
CN

347C2b/D4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., no DNA, 93071503, Reactivity: Anti-F5 (K99) pilus antigen of E.coli

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About This Item

UNSPSC Code:
41106514
Biological source:
Mouse x Bovine Heterohybridoma
Technique(s):
cell culture | mammalian: suitable
Myeloma:
94A1 (mouse x calf x calf 2° hybridoma)
Reactivity:
Anti-F5 (K99) pilus antigen of E.coli
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biological source

Mouse x Bovine Heterohybridoma

packaging

tube of 5 μg 93071503-DNA-5UG, pkg of vial of cells 93071503-1VL

ImmunoDon

Jersey calf lymph node

Myeloma

94A1 (mouse x calf x calf 2° hybridoma)

Reactivity

Anti-F5 (K99) pilus antigen of E.coli

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Immunogen

Purified pilus antigen of F5 (K99+) enterotoxigenic E.coli

Application

The isotype of the monoclonal antibody produced by this cell line is bovine IgG1.

Biochem/physiol Actions

Mouse x Bovine Jersey calf lymph node
The antibody is directed against the F5 (K99) pilus antigen of E. coli.

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.

Regulatory Information

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