AMR human cell line
IHW Number: 9206, 94022521
Synonym(s):
AM
biological source
human blood
ethnicity
English
gender
male
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Looking for similar products? Visit Product Comparison Guide
General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
A: 0205 3201
Cw: 0701 0304
B: 4901 4001
Cw: 0701 0304
B: 4901 4001
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
HLA A: 2 32
HLA C: 7 10
HLA B: 49 60
Bw4/bw6: 4 6
HLA DR: 4
HLA DR53: 53
HLA DQ: 8
HLA C: 7 10
HLA B: 49 60
Bw4/bw6: 4 6
HLA DR: 4
HLA DR53: 53
HLA DQ: 8
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service