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About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG1
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0
Reactivity:
Anti-myeloid antigen 55kDa, rat
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 95110627-DNA-5UG, pkg of vial of cells 95110627-1VL
ImmunoDon
Mouse spleen cells
Myeloma
Sp2/0
Reactivity
Anti-myeloid antigen 55kDa, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Wistar rat spleen
Application
Immunohistochemistry, cytochemistry, FACS
Biochem/physiol Actions
Not specified
The ED17 hybridoma cell line secretes antibodies reactive against a 55 kDa membrane antigen on tissue macrophages. ED17 reacts strongly with red pulp macrophages in the spleen and Langerhans cells in the skin as well as peritoneal macrophages. The antibody also recognises rat SIRP (personal communication).
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C
RPMI 1640 + 2 mM glutamine + 10% FBS
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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