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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
cGMP Enzyme Immunoassay Kit, Direct, sufficient for 96 assays
usage
sufficient for 96 assays
technique(s)
ELISA: suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... PRKG1(5592), PRKG2(5593)
Application
Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
target_organs
Respiratory system
supp_hazards
Storage Class
3 - Flammable liquids
flash_point_f
120.2 °F
flash_point_c
49 °C
Regulatory Information
低风险生物材料
危险化学品
常规特殊物品
易制毒化学品(2类)
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Cardiac hypertrophy is a major risk factor for morbidity and mortality in a number of cardiovascular diseases. Consequently, the signaling pathways that inhibit cardiac hypertrophy are currently receiving much interest. Among them, nitric oxide (NO), signaling via cGMP and cGMP-dependent
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We previously reported NO/sGC signaling in the upper respiratory pathway, receiving input from the respiratory neurons of the brainstem to phrenic motoneurons in the C3-C6 spinal cord. In order to assess whether innervation of the neuromuscular junction (NMJ) at the
Rachael P Norris et al.
Reproduction (Cambridge, England), 140(5), 655-662 (2010-09-10)
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