DUO82064
Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
Synonym(s):
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
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About This Item
product line
Duolink®
Quality Level
technique(s)
proximity ligation assay: suitable
fluorescence
λex 360 nm; λem 460 nm
suitability
suitable for fluorescence-detection automated sequencing
suitable for microtiter plates
shipped in
dry ice
storage temp.
−20°C
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This Item | DUO92007 | DUO82040 | DUO92010 |
|---|---|---|---|
| technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable |
| suitability suitable for fluorescence-detection automated sequencing, suitable for microtiter plates | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for brightfield, suitable for fluorescence |
| fluorescence λex 360 nm; λem 460 nm | fluorescence λex 554 nm; λem 576 nm (Cyanine 3; Zeiss Filter set 20) | fluorescence λex 360 nm; λem 460 nm (Zeiss Filter set 49) | fluorescence - |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Application
Use the Multiwell Plates modifications to the Duolink® In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Duolink® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink® In Situ in microtiter plates. See the datasheet for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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