DUO90806
Duolink® ImageTool
technique(s)
proximity ligation assay: suitable
storage temp.
20-25°C
Application
Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA® probes, one PLUS and one MINUS, your choice of Detection Reagents. Recommended reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using Duolink ImageTool.
Duolink ImageTool is a dedicated and user-friendly software, specifically designed for objective quantification/counting of PLA signals in images generated from fluorescence microscopy. Both cells and tissue images may be analyzed. The nuclei are automatically detected and cytoplasm size estimated, enabling single cell statistical analysis of expression levels in tissue or cell populations. Furthermore regions of interest can be defined, a feature of particular relevance when studying tissue samples. Raw imaging data can be imported directly from the four major microscope vendors (Olympus, Leica, Nikon and Zeiss) as well as tiff and jpg. The results data can easily be exported to Microsoft Excel for further evaluation.
Demo version
When running the demo version you can import your own images and use all features of the software except getting the report of the analysis. To run the software in full mode you need a USB flash drive that works as a software protection key.
Download Demo Version
Duolink ImageTool Tutorial
Duolink ImageTool User Manual
Duolink ImageTool Test Images
Find answers to commonly asked question on our Duolink FAQ page
To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA® probes, one PLUS and one MINUS, your choice of Detection Reagents. Recommended reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using Duolink ImageTool.
Duolink ImageTool is a dedicated and user-friendly software, specifically designed for objective quantification/counting of PLA signals in images generated from fluorescence microscopy. Both cells and tissue images may be analyzed. The nuclei are automatically detected and cytoplasm size estimated, enabling single cell statistical analysis of expression levels in tissue or cell populations. Furthermore regions of interest can be defined, a feature of particular relevance when studying tissue samples. Raw imaging data can be imported directly from the four major microscope vendors (Olympus, Leica, Nikon and Zeiss) as well as tiff and jpg. The results data can easily be exported to Microsoft Excel for further evaluation.
Demo version
When running the demo version you can import your own images and use all features of the software except getting the report of the analysis. To run the software in full mode you need a USB flash drive that works as a software protection key.
Download Demo Version
Duolink ImageTool Tutorial
Duolink ImageTool User Manual
Duolink ImageTool Test Images
Find answers to commonly asked question on our Duolink FAQ page
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Maneka Chitiprolu et al.
Nature communications, 9(1), 2794-2794 (2018-07-20)
Mutations in proteins like FUS which cause Amyotrophic Lateral Sclerosis (ALS) result in the aberrant formation of stress granules while ALS-linked mutations in other proteins impede elimination of stress granules. Repeat expansions in C9ORF72, the major cause of ALS, reduce
Thomas W Bonagura et al.
Endocrinology, 153(6), 2897-2906 (2012-04-13)
We previously showed that advancing the increase in estradiol levels from the second to the first third of baboon pregnancy suppressed placental extravillous trophoblast (EVT) invasion and remodeling of the uterine spiral arteries. Cell culture studies show that vascular endothelial
Jaclyn J Renfrow et al.
Neuro-oncology, 13(8), 880-885 (2011-07-30)
We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein
Charles Lu et al.
PloS one, 7(4), e34833-e34833 (2012-05-05)
Tumor suppressor gene TUSC2/FUS1 (TUSC2) is frequently inactivated early in lung cancer development. TUSC2 mediates apoptosis in cancer cells but not normal cells by upregulation of the intrinsic apoptotic pathway. No drug strategies currently exist targeting loss-of-function genetic abnormalities. We
Florian Alonso et al.
American journal of physiology. Heart and circulatory physiology, 299(5), H1365-H1373 (2010-08-31)
Upon agonist stimulation, endothelial cells trigger smooth muscle relaxation through the release of relaxing factors such as nitric oxide (NO). Endothelial cells of mouse aorta are interconnected by gap junctions made of connexin40 (Cx40) and connexin37 (Cx37), allowing the exchange
Related Content
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service