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E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

Name: Ethylenediaminetetraacetic acid disodium salt dihydrate
Brand: SIGMA

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂, Sequestrene Na₂

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₄N₂Na₂O₈ · 2H₂O

CAS Number:

6381-92-6

Molecular Weight:

372.24

NACRES:

NA.31

PubChem Substance ID:

24894605

UNSPSC Code:

12352107

EC Number:

205-358-3

Beilstein/REAXYS Number:

3900609

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grade

Molecular Biology

Quality Level

300

assay

99.0-101.0% (titration)

form

powder

reaction suitability

reagent type: chelator

technique(s)

electrophoresis: suitable

impurities

≤0.005% Insolubles, ≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3 M NaOH: 1.6 g/10 mL

cation traces

Fe: ≤0.005%, Pb: ≤0.002%

suitability

suitable for electrophoresis, suitable for molecular biology

foreign activity

DNase, RNase, NICKase and protease, none detected

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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Related Categories

Biochemicals

Biological Buffers

Chelating & Reducing Agents for Research

General description

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

Application

EDTA disodium salt has been used in research:

as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
an anticoagulant for whole blood collection
a stabilizer solution for freezing cell culture samples
in combination with PBS and trypsin, to trypsinize cells
for phytoextraction of heavy metals in polluted soils

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Features and Benefits

Molecular Biology grade, suitable for many molecular biology applications
Free of endonucleases DNase, RNase, Nickase, and Protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

GHS07,GHS08

signalword

Warning

hcodes

H332 - H373

pcodes

P260 - P271 - P304 + P340 + P312 - P314 - P501

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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External quality assessment of methylmalonic acid and total homocysteine

Clinical Chemistry, 45, 1536-1542 (1999)

Chelant-enhanced heavy metal uptake by Eucalyptus trees under controlled deficit irrigation

Fine P et al.

The Science of the Total Environment, 493, 995-1005 (2014)

Myeloperoxidase Inhibition Decreases the Expression of Collagen and Metallopeptidase in Mare Endometria under In Vitro Conditions.

Ana Amaral et al.

Animals : an open access journal from MDPI, 11(1) (2021-01-21)

Neutrophils can originate neutrophil extracellular traps (NETs). Myeloperoxidase (MPO) is a peroxidase found in NETs associated to equine endometrosis and can be inhibited by 4-aminobenzoic acid hydrazide (ABAH). Metallopeptidases (MMPs) participate in extracellular matrix stability and fibrosis development. The objectives

External quality assessment of methylmalonic acid and total homocysteine.

M?ller J, et al.

Clinical Chemistry, 45(9), 1536-1542 (1999)

Expression of ABCG2 and Bmi-1 in oral potentially malignant lesions and oral squamous cell carcinoma.

Dalley AJ, et al.

Cancer Medicine, 3(2), 273-283 (2014)

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蛋白相互作用

本协议描述了一种使用甲醛完成化学交联蛋白质的方法。除零长交联剂外，甲醛是交联跨度最短（~2-3 Å）的交联剂，因此使其成为非常可靠地检测特定蛋白质-蛋白质相互作用的理想工具。

Global Trade Item Number

SKU	GTIN
E5134-100G	04061835544509
E5134-5KG	04061835544547
E5134-50G	04061835544530
E5134-250G	04061835544523
E5134-1KG	04061835544516
E5134-500G	04061838357540

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