E5134
Ethylenediaminetetraacetic acid disodium salt dihydrate
suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)
Synonym(s):
Sequestrene Na2, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2
grade
Molecular Biology
Quality Level
Assay
99.0-101.0% (titration)
form
powder
reaction suitability
reagent type: chelator
technique(s)
electrophoresis: suitable
impurities
≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)
mp
248 °C (dec.) (lit.)
solubility
3 M NaOH: 1.6 g/10 mL
cation traces
Fe: ≤0.005%
Pb: ≤0.002%
suitability
suitable for electrophoresis
suitable for molecular biology
foreign activity
DNase, RNase, NICKase and protease, none detected
storage temp.
room temp
SMILES string
O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
InChI key
OVBJJZOQPCKUOR-UHFFFAOYSA-L
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General description
Application
- as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
- an anticoagulant for whole blood collection
- a stabilizer solution for freezing cell culture samples
- in combination with PBS and trypsin, to trypsinize cells
- for phytoextraction of heavy metals in polluted soils
Features and Benefits
- Molecular Biology grade, suitable for many molecular biology applications
- Free of endonucleases DNase, RNase, Nickase, and Protease
Other Notes
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can solutions of Product E5134, EDTA disodium salt, be autoclaved?
Yes.
Is Product E5134, EDTA disodium salt, stable in solution?
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.
What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?
The optimal concentration is 1.5 mg per mL of blood.
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Protocols
Formaldehyde cross-linking protocol detects specific protein interactions with confidence.
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明
本协议描述了一种使用甲醛完成化学交联蛋白质的方法。除零长交联剂外，甲醛是交联跨度最短（~2-3 Å）的交联剂，因此使其成为非常可靠地检测特定蛋白质-蛋白质相互作用的理想工具。
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