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Merck
CN

G9669

Sigma-Aldrich

Anti-GABAA Receptor (β3 subunit), Cytosolic Loop antibody produced in rabbit

buffered aqueous glycerol solution, affinity isolated antibody

Synonym(s):

Anti-γ-Aminobutyric Acid Type A Receptor (β3 subunit)

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About This Item

MDL number:
UNSPSC Code:
12352203
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 50-56 kDa

species reactivity

rat

technique(s)

western blot: 1:1000 using rat brain membrane fractions

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Gabrb3(24922)

General description

GABAA and GABAB receptors differ with regard to their ionic characteristics and pharmacological properties. The GABAA receptor is an ionotropic receptor that forms the GABA gated chloride channel and consists of several heterogeneous subunits with membrane recognition sites for benzodiazapenes. GABAA receptor is a ligand-gated chloride channel that is a multimeric subunit complex. So far, around 16 subunits have been identified. This gene encodes the β 3 subunit, which maps to human chromosome 15q11-13. Three human GABAA receptor subunit genes, GABRB3, GABRA5, and GABRG3, are expressed exclusively from the paternal allele.

Immunogen

fusion protein with the amino acid sequence representing the cytosolic loop of the rat GABAA receptor (β3 subunit).

Application

Anti-GABAA Receptor (β 3 subunit), Cytosolic Loop antibody produced in rabbit is suitable for western blotting at a working dilution of 1:1000 using rat brain membrane fractions.

Biochem/physiol Actions

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. 155CA-2, a marker of GABRB3 has been associated with autistic disorder. Mutations in the β-3 subunit gene has also been linked to insomnia. The function of GABA receptor is inhibited by zinc ions based on the receptor subunit composition. The sites that mediate zinc inhibition are β-3 His267 and Glu270 located within the ion channel, and two more on the external N-terminal face of the receptor.

Physical form

Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Alastair M Hosie et al.
Nature neuroscience, 6(4), 362-369 (2003-03-18)
Zinc ions are concentrated in the central nervous system and regulate GABA(A) receptors, which are pivotal mediators of inhibitory synaptic neurotransmission. Zinc ions inhibit GABA(A) receptor function by an allosteric mechanism that is critically dependent on the receptor subunit composition:
J D Buxbaum et al.
Molecular psychiatry, 7(3), 311-316 (2002-03-29)
Autistic disorder (OMIM 209850) is a disease with a significant genetic component of a complex nature.(1) Cytogenetic abnormalities in the Prader-Willi/Angelman syndrome critical region (15q11-13) have been described in several individuals with autism.(1) For this reason, markers across this region
Andreas Buhr et al.
Human genetics, 111(2), 154-160 (2002-08-22)
We screened 124 individuals for single nucleotide polymorphisms of the alpha1, beta3 and gamma2 genes of the GABA(A) receptor in the regions corresponding to the ligand-binding domains on the protein level. In a patient with chronic insomnia, a missense mutation
M Meguro et al.
Human molecular genetics, 6(12), 2127-2133 (1997-11-05)
We have constructed mouse A9 hybrids containing a single normal human chromosome 15, via microcell-mediated chromosome transfer. Cytogenetic and DNA-polymorphic analyses identified mouse A9 hybrids that contained either a paternal or maternal human chromosome 15. Paternal specific expression of the
P J McLean et al.
Genomics, 26(3), 580-586 (1995-04-10)
We demonstrated previously that an alpha 1-beta 2-gamma 2 gene cluster of the gamma-aminobutyric acid (GABAA) receptor is located on human chromosome 5q34-q35 and that an ancestral alpha-beta-gamma gene cluster probably spawned clusters on chromosomes 4, 5, and 15. Here

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