GUSA
β-Glucuronidase Fluorescent Activity Detection Kit
usage
kit sufficient for 200 standard tests
shipped in
dry ice
storage temp.
−20°C
Application
For quantitative assay of β-glucuronidase gene products in plant or animal cell lysates.
Features and Benefits
- Fluorescent detection for quantitative and qualitative measurement
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Other Notes
The substrate used in this kit is MU-GlcA (4-methylumbelliferyl β-D-glucuronide), a widely used fluorogenic substrate for determination of glucuronidase activity.
Kit Components Only
Product No.
Description
- Detailed technical bulletin 1 ea
Kit Components Also Available Separately
Product No.
Description
SDS
- 5× extraction buffer 25 mL
- β-Glucuronidase 1000 U
- M15084-MU 25 mg
- 4-MU-GlcA 25 mg
- Stop solution 100 mL
Regulatory Information
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An improved assay for beta-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous beta-glucuronidase activity. .
Kosugi, S., et al.
Plant Science, 70, 133-140 (1990)
Current Protocols in Molecular Biology, 9-9 (1996)
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R.A., et al.
The Embo Journal, 6, 390-3907 (1987)
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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General Information
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