KEM0014
Poly(A) Polymerase
Ultra-pure enzyme for nucleic acid modifications
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grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
>20,000 U/mg
concentration
5,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
Application
Suitable for:
- Labeling of RNA with ATP or cordyceptin
- Poly(A) tailing of RNA for cloning or affinity purification
- Enhancing translation or RNA transferred into eukaryotic cells
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 25 mM Tris-HCl, 500 mM NaCl, 1 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 8.0 @ 25° C.
Other Notes
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-soluble material in 10 minutes at 37° C.
Source of protein: The gene encoding E. coli Poly(A) Polymerase expressed from a plasmid in E coli.
Supplied with:KEM0014B (Poly(A) Polymerase Reaction Buffer)KEM0070B (10 mM ATP solution)
Unit size: 1,000 U
Storage Class
10 - Combustible liquids
Regulatory Information
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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing