M1814
MKK7 α1, active human
>70% (by Coomassie blue staining following SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
MAP kinase kinase 7 α1
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About This Item
UNSPSC Code:
12352200
MDL number:
recombinant
expressed in E. coli
assay
>70% (by Coomassie blue staining following SDS-PAGE)
form
buffered aqueous solution
mol wt
65 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MAP2K7(5609)
Application
May be used for protein phosphorylation assay.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 0.1% (v/v) 2-mercaptoethanol, 0.1 mM benzamidine, and 0.2 mM PMSF.
Preparation Note
Affinity purified using glutathione-agarose and activated with MEK kinase.
Other Notes
1 unit of active MKK7α1 will transfer 1 nmole of phosphate to ATF2 per minute at 30 °C.
GST fusion protein encoding the last 333 residues of human MKK7α1
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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