M5815
Monoclonal Anti-Myogenin antibody produced in mouse
clone F12B, purified immunoglobulin, buffered aqueous solution
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About This Item
Conjugate:
unconjugated
Clone:
F12B, monoclonal
Application:
ELISA (i), IF, IHC (p), IP, WB
Citations:
16
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
F12B, monoclonal
form
buffered aqueous solution
mol wt
antigen 34 kDa
species reactivity
mouse, rat, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections, immunoprecipitation (IP): 2 μg using 1 mg protein lysate, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1 μg/mL
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)
General description
Myogenin belongs to the basic helix-loop-helix (bHLH) superfamily.
Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.
Immunogen
rat recombinant myogenin (amino acids 30-224).
Application
Monoclonal Anti-Myogenin antibody produced in mouse has been used in immunohistochemistry, immunofluorescence and western blotting.
Biochem/physiol Actions
Myogenin acts as a biomarker in rhabdomyosarcoma (RMS). It has a role in developmental processes, such as hematopoiesis, neurogenesis, myogenesis, tumor cell invasion and metastasis.
Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.
Physical form
Solution in phosphate buffered saline containing 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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