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Merck
CN

M7556

Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

Synonym(s):

Anti-ERK-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44
MDL number:
MFCD01633614
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
4

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5 μg using 0.5-1 mg of a mouse 3T3 fibroblast lysate, western blot: 0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells

UniProt accession no.

P28482

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MAPK1(5594)
mouse ... Ephb2(13844), Mapk1(26413)
rat ... Mapk1(116590)

General description

Mitogen-activated protein kinases (MAPKs) regulate a wide range of functions by modulating cellular signal transduction. These functions include cellular growth, differentiation, movement and apoptosis. Conventional MAPKs comprise of the ERK1/2, p38, JNK and ERK5 kinase families. ERK1 and ERK2 are highly conserved serine/threonine kinases that are responsive to growth factors
Anti-MAP Kinase 2 (ERK-2) antibody is specific for MAPK2 (42 kDa) in mice, humans and rats. This antibody does not cross react with MAP kinase 1 (ERK-1) nor does it interfere with the kinase activity.

Immunogen

recombinant mouse MAP Kinase 2 (MAPK2, ERK-2).

Application

Anti-MAP Kinase 2 (ERK-2) antibody is suitable for use in western blot (0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells). The product can also be used for immunoprecipitation (5 μg, using 0.5-1 mg of a mouse 3T3 fibroblast lysate).

Physical form

Solution in 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and ≤ 0.1% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN1350
SLBT2972
SLBK4144
SLBK4144V
039K1058

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H Owaki et al.
Biochemical and biophysical research communications, 182(3), 1416-1422 (1992-02-14)
Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to
Marie Cargnello et al.
Microbiology and molecular biology reviews : MMBR, 75(1), 50-83 (2011-03-05)
The mitogen-activated protein kinases (MAPKs) regulate diverse cellular programs by relaying extracellular signals to intracellular responses. In mammals, there are more than a dozen MAPK enzymes that coordinately regulate cell proliferation, differentiation, motility, and survival. The best known are the
Yusaku Miyamae et al.
Cell chemical biology, 27(12), 1573-1581 (2020-10-03)
Here, we report a method to regulate cellular protein levels by introducing a ubiquitin variant between a destabilizing domain (DD) and the regulated protein. When produced in the absence of a stabilizing ligand the DD dominates and the entire fusion
Yunjie Zhang et al.
Oncology letters, 15(4), 5620-5626 (2018-03-20)
The present study assessed the mechanism underlying the effect of sorafenib on the proliferation and apoptosis of the acute promyelocytic leukemia (APL) cell line NB4. NB4 cells were treated with different concentrations of sorafenib (0, 1.5, 3, 6, and 12

Related Content

Data Sheet - M7556

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