MAK566
Acetyl Coenzyme A Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Acetyl-CoA Assay Kit
Quality Level
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
Related Categories
General description
Acetyl-CoA (AcCoA) is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. AcCoA acts as an indicator of fat, sugar, and protein levels and shows up on the nutritional status. In lowered glucose conditions, AcCoA participates in the production of ATP. Increased AcCoA levels are observed in prostate cancer due to elevated fatty acid utilization and thus provides an additional energy source for the tumor cell growth.
Application
Suitable for measuring Acetyl-CoA in various biological samples such as cell lysates and tissues.
The Acetyl Coenzyme A Assay Kit may be used for:
Cancer Metabolism Research
Mitochondrial Metabolism Research
Neurological Research
Cancer Metabolism Research
Mitochondrial Metabolism Research
Neurological Research
Biochem/physiol Actions
Acetyl-CoA concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 340/λem = 460 nm) product, proportional to the Acetyl-CoA present. Typical sensitivities of detection for this kit are 20-1000 pmol of Acetyl CoA. This kit is a highly sensitive assay for determining Acetyl-CoA level in a variety of biological samples.
Features and Benefits
Enhanced Sensitivity and Accuracy: Detect Acetyl-CoA (AcCoA) concentrations ranging from 20 - 1000 pmol providing precise results across a wide range of sample types.
Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.
Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file.
Compatibility with High-Throughput Systems: Seamlessly integrate our kit into high-throughput handling systems, ensuring smooth operations and accurate results, enhancing efficiency in your laboratory workflow.
Convenient Data Analysis: Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
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Acetyl coenzyme A: a central metabolite and second messenger
Pietrocola F, et al.
Cell Metabolism, 21(6), 805-821 (2015)
Fatty acid oxidation is a dominant bioenergetic pathway in prostate cancer
Liu Y, et al.
Prostate Cancer and Prostatic Diseases, 9(3), 230-234 (2006)
Y Liu
Prostate cancer and prostatic diseases, 9(3), 230-234 (2006-05-10)
Most malignancies have increased glycolysis for energy requirement of rapid cell proliferation, which is the basis for tumor imaging through glucose analog FDG (2-deoxy-2-fluoro-D-glucose) with positron emission tomography. One of significant characteristics of prostate cancer is slow glycolysis and low
N J Watmough et al.
The Biochemical journal, 262(1), 261-269 (1989-08-15)
The quantitative isolation of acyl-CoA esters of chain length C2-C17 from mitochondrial incubations and their analysis by reverse-phase radio-h.p.l.c. is described. Photodiode-array detection was used to characterize 2-enoyl-CoA esters. The chromatographic behaviour of all 27 intermediates of the beta-oxidation of
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