N7265
Nitrate Reductase (NAD[P]H) from Aspergillus niger
lyophilized powder, ≥300 units/g solid
Synonym(s):
NAD(P)H:Nitrate oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Nitrate Reductase (NAD[P]H) from Aspergillus niger, lyophilized powder, ≥300 units/g solid
form
lyophilized powder
specific activity
≥300 units/g solid
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Biochem/physiol Actions
Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.
Disclaimer
light and moisture sensitive
General description
Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.
Other Notes
One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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J Miyazaki et al.
Molecular & general genetics : MGG, 228(3), 329-334 (1991-09-01)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from
P C Allen
Poultry science, 76(6), 810-813 (1997-06-01)
The objective of this study was to gather evidence for production of nitric oxide (NO) during a primary infection with the protozoan parasite Eimeria tenella, which carries out its life cycle in the ceca of chickens. Relationships of plasma levels
W H Campbell
Cellular and molecular life sciences : CMLS, 58(2), 194-204 (2001-04-06)
Pyridine nucleotide-dependent nitrate reductases (NRs; EC 1.6.6.1-3) are molybdenum-containing enzymes found in eukaryotic organisms which assimilate nitrate. NR is a homodimer with an approximately 100 kDa polypeptide which folds into stable domains housing each of the enzyme's redox cofactors--FAD, heme-Fe
Purification and properties of assimilatory nitrate reductase [NAD(P)H] from Ankistrodesmus braunii.
M A de la Rosa et al.
European journal of biochemistry, 106(1), 249-256 (1980-05-01)
Assimilatory nitrate reductase [NAD(P)H] (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by a simple procedure that utilizes as the main step affinity chromatography on Blue-Sepharose. The best enzyme preparation has a specific activity of 61.25 units/mg protein.
G Unden et al.
Molecular microbiology, 25(2), 205-210 (1997-07-01)
The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The
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