P0085
Anti-Podoplanin antibody, RAT monoclonal
clone NZ-1.2, purified from hybridoma cell culture
Synonym(s):
Anti-AGGRUS, Anti-GP36, glycoprotein, 36-KD, Anti-GP40, Anti-Gp38, Anti-OTS8, Anti-T1A, Anti-T1A-2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NZ-1.2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~36 kDa
species reactivity
human
concentration
~1.5 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 4-8 μg/mL using U87 total cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDPN(10630)
Related Categories
General description
Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stimulating (PLAG) domain of podoplanin. Podoplanin (Aggrus) is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has been described to be present in normal, inflammatory and neoplastic lymphatic capillary endothelium.
Immunogen
platelet-aggregation-stimulating (PLAG) domain of human podoplanin.
Application
Anti-Podoplanin antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- flow cytometry
- immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor
Kaneko MK, et al.
Febs Letters, 581(2), 331-336 (2007)
Peter Birner et al.
Cancer, 120(16), 2440-2447 (2014-04-29)
Recently, isocitrate dehydrogenase 1 (IDH1) was identified as a major participant in glioma pathogenesis. At present, the enzymatic activity of the protein has been the main topic in investigating its physiological function, but its signaling pathway allocation was unsuccessful. Interestingly
Hao Xue et al.
Autophagy, 12(7), 1129-1152 (2016-05-11)
Hypoxia induces protective autophagy in glioblastoma cells and new therapeutic avenues that target this process may improve the outcome for glioblastoma patients. Recent studies have suggested that the autophagic process is upregulated in glioblastomas in response to extensive hypoxia. Hypoxia
The potential role of podoplanin in tumour invasion
Wicki A and Christofori G
British Journal of Cancer, 96(1), 1-1 (2007)
S Breiteneder-Geleff et al.
The American journal of pathology, 151(4), 1141-1152 (1997-11-05)
Puromycin aminonucleoside nephrosis (PAN), a rat model of human minimal change nephropathy, is characterized by extensive flattening of glomerular epithelial cell (podocyte) foot processes and by severe proteinuria. For comparison of expression of glomerular membrane proteins of normal and PAN
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