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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

form

DMSO solution

Quality Level

packaging

pkg of 1 ml, 5 ml ×

shipped in

wet ice

storage temp.

−20°C

General description

Protease and Phosphatase Inhibitor Cocktail us designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidases. Protease and Phosphatase Inhibitor Cocktail also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificate of Analysis

Certificate of Origin

Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers
Quitterer U, et al.
Cell, S0092-8674(18), 31406-31406 (2018)
Astrocytes resolve ER stress through mitochondrial fusion facilitated by biotin availability.
Anand, et al.
Cell Stress & Chaperones, 25, 945-953 (2021)
Mechanisms of adipose tissue extracellular matrix alterations in an in vitro model of adipocytes hypoxia and aging.
Zoico, et al.
Mechanisms of Ageing and Development, 192, 111374-111374 (2021)
null
Graphene quantum dots prevent alpha-synucleinopathy in Parkinson?s disease
Kim D, et al.
Nature Nanotechnology, 13(9), 812-812 (2018)

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