S1826
Sialic Acid Aldolase from Escherichia coli K12
recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein
Synonym(s):
N-Acetylneuraminate lyase, N-Acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming)
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About This Item
Specific activity:
≥3.0 units/mg protein
Recombinant:
expressed in E. coli BL21
recombinant
expressed in E. coli BL21
Quality Segment
form
lyophilized powder
specific activity
≥3.0 units/mg protein
mol wt
33.4 kDa
shipped in
dry ice
storage temp.
−20°C
General description
Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.
Application
Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.
Physical form
Lyophilized powder containing Tris-HCl and NaCl
Analysis Note
Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 7.5) containing Neu-5-Ac (10 mM) at 37 °C for 15 min and analyzed using capillary electrophoresis with UV detection at 200 nm.
Other Notes
One unit will catalyze the formation of 1.0 μmol Neu-5-Ac from Man-N-Ac and pyruvate per minute at 37°C at pH 8.0.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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