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Merck
CN

SAB5600227

Anti-His-Tag antibody, Rabbit monoclonal

recombinant, expressed in HEK 293 cells, clone RM146, purified immunoglobulin

Synonym(s):

10 His epitope tag, 6 His epitope tag, HHHHHH tag, Polyhistidine Tag

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
RM146, monoclonal, recombinant monoclonal
Species reactivity:
all
Application:
FACS, ICC, IHC, IP, WB
Citations:
6
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recombinant

expressed in HEK 293 cells

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM146, monoclonal, recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

technique(s)

flow cytometry: 0.5-2 μg/mL, immunocytochemistry: 0.5-2 μg/mL, immunohistochemistry: 0.1—1 μg/mL, immunoprecipitation (IP): 0.5-2 μg/mL, western blot: 0.1— 1 μg/mL

isotype

IgG

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Biochem/physiol Actions

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Physical form

Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
常规特殊物品

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Tarah Elizabeth Trebino et al.
eLife, 12 (2023-12-27)
BRAF is a key member in the MAPK signaling pathway essential for cell growth, proliferation, and differentiation. Mutant BRAF is often the underlying cause of various types of cancer and mutant RAS, the upstream regulator of BRAF, is a driver
Human inhalable antibody fragments neutralizing SARS-CoV-2 variants for COVID-19 therapy.
Minenkova, et al.
Molecular Therapy, 30, 1979-1993 (2022)
Harman Malhi et al.
Cell reports. Medicine, 3(6), 100658-100658 (2022-06-16)
Epstein-Barr virus (EBV) is a cancer-associated pathogen responsible for 165,000 deaths annually. EBV is also the etiological agent of infectious mononucleosis and is linked to multiple sclerosis and rheumatoid arthritis. Thus, an EBV vaccine would have a significant global health