T1503
Trizma® base
Primary Standard and Buffer, ≥99.9% (titration), crystalline
Quality Level
description
aminopeptidase substrate
Assay
≥99.9% (titration)
form
crystalline
pH
10.5-12
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)
absorption
≤0.05 at 290 nm at 40%
application(s)
diagnostic assay manufacturing
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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General description
Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.
Application
- as a component of H buffer (cell dissociation buffer)
- for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
- as an assay buffer for reconstitution of extracted and dried protein samples
- to prepare Tris-HCl buffer that is used to stabilize proteins
- as a buffer to extract carotenoid from tubers
- as a component of sample buffer during protein extraction prior to western blotting
- as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
- as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
Features and Benefits
- Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Can be used in Cell Biology, and Biochemical research
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
also commonly purchased with this product
comparable product
suggested gloves for splash protection
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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What is the pH of Trizma® base, T1503?
The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.
What type of electrode should I use to measure the pH of Trizma® base T1503?
We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.
How can I use T1503 to make a Trizma® buffered solution?
Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.
Can Product T1503, Trizma® base be used as a standard?
Trizma base meets the requirements for a primary basimetric standard.
Can I autoclave solutions that contain Product T1503, Trizma® base?
Yes, Trizma buffered solutions may be autoclaved.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols
To measure carboxypeptidase A activity, hippuryl-L-phenylalanine is used in a continuous spectrophotometric rate determination assay at 254 nm. Carboxypeptidase A hydrolyzes peptide bonds at C-terminal residues.
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
To measure luciferase activity, an assay that measures light emission with a luminometer is used. Firefly luciferase is used for detection and quantitation of ATP and as a reporter for genetic function.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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