T2413
Trizma® hydrochloride solution
1 M, pH 7.0
Synonym(s):
Tris hydrochloride solution
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
SMILES string
Cl.NC(CO)(CO)CO
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
grade
Molecular Biology
sterility
0.2 μm filtered
form
liquid
concentration
1 M
impurities
DNase, RNase, NICKase and protease, none detected
pH
7.0
application(s)
diagnostic assay manufacturing
storage temp.
room temp
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Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Regulatory Information
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Anthony G Boghdadi et al.
Nature communications, 12(1), 6906-6906 (2021-11-27)
Astrocytes play critical roles after brain injury, but their precise function is poorly defined. Utilizing single-nuclei transcriptomics to characterize astrocytes after ischemic stroke in the visual cortex of the marmoset monkey, we observed nearly complete segregation between stroke and control
Chromatin isolation by RNA purification (ChIRP).
Ci C Chu, Jeffrey J Quinn, Howard Y HY Chang
Journal of Visualized Experiments, 61 (2012)
Daniel Franjic et al.
Neuron (2021-11-20)
The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer's disease. To investigate neurogenic potential and cellular diversity, we profiled single-nucleus transcriptomes in five hippocampal-entorhinal subregions in humans, macaques, and pigs.
Ci Chu et al.
Molecular cell, 44(4), 667-678 (2011-10-04)
Long noncoding RNAs (lncRNAs) are key regulators of chromatin state, yet the nature and sites of RNA-chromatin interaction are mostly unknown. Here we introduce Chromatin Isolation by RNA Purification (ChIRP), where tiling oligonucleotides retrieve specific lncRNAs with bound protein and
Susana Mingote et al.
Bio-protocol, 7(1), e2090-e2090 (2017-01-05)
Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured
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