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T2819

Trizma^® hydrochloride solution

Name: Trizma<SUP>®</SUP> hydrochloride solution
Brand: SIGMA

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

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Properties

Quality Segment

200

grade

BioPerformance Certified, Molecular Biology

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

9.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

Description

General description

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma^® hydrochloride solution has been used:

to neutralize IgG bound to protein A/G column
as a component in BigDye buffer for sequencing reaction
to block slides

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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