T2819
Trizma® hydrochloride solution
pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
grade
BioPerformance Certified
Molecular Biology
Quality Level
form
solution
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH
9.0
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
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General description
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
Application
Trizma® hydrochloride solution has been used:
- to neutralize IgG bound to protein A/G column
- as a component in BigDye buffer for sequencing reaction
- to block slides
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tris/Tris (textperiodcentered) HCl: a standard buffer for use in the physiologic pH range
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