50994-U
BIOshell Glycan (2.7 μm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
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About This Item
Product Name
BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size
material
stainless steel hardware (column)
Quality Level
Agency
suitable for USP L86
description
shell thickness (0.5 μm)
solid core (1.7 μm)
product line
BIOshell
feature
endcapped: no
analyte chemical class(es)
glycans
manufacturer/tradename
BIOshell
packaging
1 ea of
parameter
1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
15 cm × 2.1 mm
surface area
135 m2/g
matrix
spherical silica particle platform
superficially porous particle
matrix active group
hydroxy, penta- phase
particle size
2.7 μm
pore size
90 Å
operating pH range
2-9
separation technique
hydrophilic interaction (HILIC)
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General description
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Application
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Articles
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.
Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.
Protocols
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Related Content
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
生物分子是活细胞产生的大分子聚合物，作为活生物体的基本组成部分执行许多生命必需的生物过程。尽管现有很多分离和分析技术可供选择，最常用的还是高效液相色谱法(HPLC)。
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