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Merck
CN

577420-U

BIOshell™ IgG 1000 Å Diphenyl (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Particle size:
2.7 μm
Matrix active group:
bonding phase, Diphenyl (diphenylmethyl)
Pore size:
1000 Å pore size
Matrix:
Fused-Core® particle platform, superficially porous particle

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Product Name

BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column, column L × I.D. 10 cm × 2.1 mm

material

stainless steel column

agency

suitable for USP L11

feature

endcapped

packaging

1 ea of

extent of labeling

1.0% loading

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

column L × I.D.

10 cm × 2.1 mm

surface area

22 m2/g

matrix

Fused-Core® particle platform, superficially porous particle

matrix active group

bonding phase, Diphenyl (diphenylmethyl)

particle size

2.7 μm

pore size

1000 Å pore size

operating pH range

2-9

separation technique

reversed phase

product line

BIOshell™

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General description

BIOshell™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.

Application

UHPLC Analysis of Reduced IgG1 on BIOshell™ IgG 1000 Å Diphenyl
UHPLC Analysis of IgG2 on BIOshell™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

Analysis Note

Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USEDP001274
USEDP001273
USEDP001272
USEDP001271
USEDP001270

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