Res., 206, 261-275 (1993)
5. Sage, H. et al., J. Cell Physiol., 127, 373-387 (1986)
6. Sage, H. et al., J. Cell Biol., 109, 341-356 (1989)
7. a. Hasselaar, P. et al., J. Biol. Chem., 266,
13278-13184
(1985).
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
(1985).
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
19
vial, spin down the solid to the bottom by a microcentrifuge or by a desktop centrifuge.
2. Add 109 μl of high-purity DMSO to one vial of Dye to make a 1 mM stock solution.
3. Dilute the stock
,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼74 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 81–109 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
C (see
Appendix A, Recipes).
Chill 100% and 70% ethanol solutions at -20°C.
Equilibrate a heating block or water bath at 65°C
Sample Preparation:
From a 6- or 12-well microtiter plate choose an
occlusion-negative
Anti-Paip2 is produced in rabbit using as immunogen a
synthetic peptide corresponding to amino acids 93-109
of human Paip2 (GeneID: 51247), conjugated to KLH
via an added cysteine residue. The immunizing peptide
fluid by Protein A chromatography.
Monoclonal Anti-Prion Protein recognizes amino acid
residues 109-112 of PrP from human, hamster and
feline by immunoblotting, ELISA, immunoprecipitation
and immunohistochemistry
least 6 months at -20 °C. A stock solution containing at
least 20 µg protein per ml can be stored under sterile
conditions at 2-8 °C for one month and in single-use
aliquots for six months at −70 °C without
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. Mlinar, Diana, "The Role of Trappin-2 and RANTES
in Mediating Resistance to HIV-1 Infection
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. O'Reilly, Isobel, "Potentiation of Drug-Induced
Cytotoxicity by Conjugated Linoleic
pp. 1552-1566 (1961).
5. Fariss, M.W., and Reed, D.J., Methods Enzymol.,
143, 101-109 (1987).
6. Lorusso, M. et al., FEBS Lett., 195(1-2), 298-302
(1986).
7. Pontremoli, S. et al., J
Code PROT-RA). 109 µg of protein was loaded
on a 7 cm, pH 4-7 IPG strip (Product Code I 2906),
focused for 50,000 volt hours, then run on a 4-20%
Tris-Glycine SDS-PAGE gel at 150 volts for 70 min. The
gel
3rd ed. (Rose, N.R.
et al., eds.). American Society for Microbiology
(Washington, D.C.), pp. 99-109 (1986).
4. Rasmussen, Mads Skytte, "Characterization of
Matrix Derived Effects on Osteoclast
(Washington, D.C.), pp. 99-109 (1986).
5. Bauerle, W. L. et al., J. Amer. Soc. Hort. Sci.,
131(2), 295-301 (2006).
6. Dahabreh, Z. et al., World J. Stem Cells, 6(4),
497-504 (2014).
7. Ásgeirsson
5-8
Human
PDGF-BB is a 24.3 kDa B chain homodimer protein
containing 109 amino acid residues.
In platelets, 70% of the PDGF is present as the
AB dimer, with most of the remainder as