infection with MISSION TRC shRNA
lentiviral particle target set NM_002752
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110
p
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TRCN0000000943 TRCN0000000944
for the gp 60/110 envelope glycoprotein of HHV6.
CELLULAR LOCALIZATION: Cytoplasmic and membrane
STRAIN RECOGNITION: Types A & B
ISOTYPE: IgG2b
APPLICATIONS: Western Blot 1:10 - 1:50
Immunofluorescence
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K
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A549 HepG2 HCT116 Panc1 Hela
Expression of JNK2 after infection with MISSION TRC shRNA
lentiviral particle target set NM_002752
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Pe
rc
nM for competition studies.
Unlabeled ligand: S(–)-Propranolol (P-110) at a
final concentration of 1 µM.
3. Incubation time 60 minutes at 27 °C.
4. Separation Over GF/C filter (5 mm diam.,
presoaked
optimal working concentration by titration test.
References
1. Paulin-Levasseur, M., et al., The 2G2 antibody
recognizes an acidic 110-kDa human mitochondrial
protein. Histochem. J., 30(9), 617-625
by staurosporine in
RBL-2H3 (EC50 = 14.2 nM) and PANC-1
(EC50 = 13.9 nM) cell lines
References
1. Hug, H., et al., Rhodamine 110-linked
amino acids and peptides as substrates
to measure caspase