Results
Escherichia coli 8739 10-100 20-24 h at 33-35 °C
good growth; pronounced
turbidity
Salmonella
Typhimurium
13311 10-100 20-24 h at 33-35 °C
good growth; pronounced
turbidity
Results
Escherichia coli 8739 10-100 20-24 h at 33-35 °C
good growth; pronounced
turbidity
Salmonella
Typhimurium
13311 10-100 20-24 h at 33-35 °C
good growth; pronounced
turbidity
Results
Escherichia coli 8739 10-100 20-24 h at 33-35 °C good growth; pronounced
turbidity
Pseudomonas
aeruginosa
9027 10-100 20-24 h at 33-35 °C good growth; pronounced
turbidity
Staphylococcus
min at 121 °C, disinfect, incinerate etc.).
Quality Control
Control Strains ATCC # Inoculum CFU Incubation Expected Results
Staphylococcus
aureus
6538 10-100 20-24 h at 33-35 °C
Brauwelt, 121, 1778 (1981)
3. M. Nishikawa, M. Kohgo, Master Brew Am Association Q22-61 (1985)
4. I.E. Alcamo, Fundamentals of Microbiology, 6th Ed., Jones and Bartlett Publishers (2001)
5. L. Jespersen
agar-agar in 1 l demineralized water. Mix in 33 ml/l of a 0.1 % aqueous solution of neutral red, adjust
the pH to 7.0 and autoclave (15 min at 121 °C). Cool to 45-50 °C, add approximately 35
Pseudomonas aeruginosa 9027 10-100 20-24 h at 33-35 °C 70-200 %
Bacillus subtilis 6633 10-100 20-24 h at 33-35 °C 70-200 %
Candida albicans 10231 10-100 20-24 h at 33-35 °C 70-200 %
Pseudomonas aeruginosa 9027 10-100 20-24 h at 33-35 °C 70-200 %
Bacillus subtilis 6633 10-100 20-24 h at 33-35 °C 70-200 %
Candida albicans 10231 10-100 20-24 h at 33-35 °C 70-200 %
autoclave
for 20 min at 121 °C, disinfect, incinerate etc.).
Quality Control
Control Strains ATCC # Incubation Expected Results
Geotrichum candidum 1240 (DSM #) 5 days at 28 °C Growth good
Escherichia coli 8739 10-100 24-28 h at 33-35 °C 70-200 %
Pseudomonas aeruginosa 9027 10-100 24-28 h at 33-35 °C 70-200 %
Bacillus subtilis 6633 10-100 24-28 h at 33-35 °C 70-200 %
Burkholderia
Pseudomonas aeruginosa 9027 10-100 20-24 h at 33-35 °C 70-200 %
Bacillus subtilis 6633 10-100 20-24 h at 33-35 °C 70-200 %
Candida albicans 10231 10-100 20-24 h at 33-35 °C 70-200 %
Brauwelt, 121, 1778 (1981)
3. M. Nishikawa, M. Kohgo, Master Brew Am Association Q22-61 (1985)
4. I.E. Alcamo, Fundamentals of Microbiology, 6th Ed., Jones and Bartlett Publishers (2001)
5. L. Jespersen
Suspend 18.5 g in 500 ml of purified water. Heat to boiling to
dissolve completely. Autoclave at 121°C for 15 minutes.
Cool the medium to 45-50°C and aseptically add the contents
of 1 vial OGYE Selective
No. TN1355EN00
Preparation
Dissolve 38 g/l. Dispense into test tubes. Autoclave (15 min at 121 °C). Cool. If required add 0.02 g
Polymyxin B/l in form of an aqueous solution and mix.
The
results
Escherichia coli
ATCC 8739
20-24 h at 33-35 °C
good growth;
pronounced turbidity
Pseudomonas aeruginosa
ATCC 9027
20-24 h at 33-35 °C
good growth;
pronounced turbidity
Staphylococcus
the medium completely. Sterilize by
autoclaving at 15 lbs. pressure (121 °C) for 15 minutes.
Cool to 50 °C and asceptically add 5% (v/v) sterile
defibrinated blood. Mix well and pour into sterile petri
,000
44-48h at 33-35 °C no growth
Pseudomonas
aeruginosa
9027
10,000-
100,000
44-48h at 33-35 °C no growth
Bacillus subtilis 6633
10,000-
100,000
44-48h at 33-35 °C
growth
Streptococcus
pyogenes 12344
100,000-
1,000,000 20-24 h at 33-35 °C no growth
Staphylococcus
aureus 6538
100,000-
1,000,000 20-24 h at 33-35 °C
growth inhibited; colorless
needlestick colonies
from Casein 5 g/l
Peptone from Meat 5 g/l
D(+)-Glucose (= Dextrose) 20 g/l
Preparation
Suspend 30 g/l. If necessary dispense into smaller vessels. Autoclave (15 min at 121 °C).
. Heat to boiling to
dissolve the medium completely. Sterilize by
autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
Cool to 50°C and aseptically add the reconstituted
contents of one vial of (O5640
Escherichia coli 8739 10-100 24-28 h at 33-35 °C 70-200 %
Pseudomonas aeruginosa 9027 10-100 24-28 h at 33-35 °C 70-200 %
Bacillus subtilis 6633 10-100 24-28 h at 33-35 °C 70-200 %
Burkholderia
Engel,
1982).
Directions:
Dissolve 40 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
Principle and Interpretation:
Yeast extract acts as source of carbon, nitrogen