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In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

HPLC Method for the Separation of Nine Key Silymarin Components of Milk Thistle

Experimental steps, coupled with prediction software, provided conditions suitable for the analysis biologically active milk thistle components.

Streamlined Electrode Fabrication: A User-Friendly Guide

Guide for fabricating NMC811 cathode electrodes, detailing procedures for optimal performance in lithium-ion batteries.

Rapid DNA Ligation Kit Protocol

Restriction Enzyme Digest Protocol

Restriction enzyme collection has been optimized for digestion using five unique buffers.

Inserting Gels into Ettan™ DALT Electrophoresis Units

This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

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