at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
at 2–8 °C
• Test Compound Stock Solution: Dissolve
compound at 200× concentration in DMSO and
vortex to mix. If necessary, warm or sonicate to
dissolve completely. Store up to 6 months
at 2–8 °C.
Fidelity
PCR System
100 U, 3.5 U/μl, 40 reactions in a final volume of 50 μl 11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume of 50 μl 11 732 650 001
2,500
Fidelity
PCR System
100 U, 3.5 U/μl, 40 reactions in a final volume of 50 μl 11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume of 50 μl 11 732 650 001
2,500
(1974).
6. Näslund, B. et al., J. Lipid. Res., 34(4), 633-641
(1993).
7. Hayes, W.K. et al., Toxicon, 31(7), 881-887
(1993).
8. Van Nieuwenhoven, F.A. et al., Circulation,
92(10), 2848-2854
Solid-phase extraction of organic
compounds: A critical review (Part I). Trends in Analytical Chemistry,
641-654. doi:http://dx.doi.org/10.1016/j.track.2015.08.015
2. Michopoulos, F. L. et al (2009). UPLC-
Solid-phase extraction of organic
compounds: A critical review (Part I). Trends in Analytical Chemistry,
641-654. doi:http://dx.doi.org/10.1016/j.track.2015.08.015
2. Michopoulos, F. L. et al (2009). UPLC-
Fidelity
PCR System
100 U, 3.5 U/μl, 40 reactions in a final volume of 50 μl 11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume of 50 μl 11 732 650 001
2,500
Fidelity PCR System 100 U, 1 x 100 U, 40 reactions in a final volume
of 50 μl
11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume
of 50 μl
Fidelity PCR System 100 U, 1 x 100 U, 40 reactions in a final volume of 50 μl 11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume of 50 μl 11 732
Fidelity PCR System 100 U, 1 x 100 U, 40 reactions in a final volume of 50 μl 11 732 641 001
500 U, 2 x 250 U, 200 reactions in a final volume of 50 μl 11 732
gelagert
werden sollen, sind sie bei 2° bis 8°C in einem sicheren Behälter in
Dunkelheit zu lagern.
8. Die Objektträger mit einem Fluoreszenzmikroskop bei 160–200-facher
Vergrößerung auf Zellen untersuchen
for 4 pulses. Lane 3: chromatin sonicated for 8 pulses.
4: chromatin sonicated for 16 pulses.
23
DNA Sonication: ChIP DNA Should Be Between 200- 1000 bp in Length
Sheared chromatin from
H M R WB APur Rb IgG 200 µL 07-339
Anti-phospho-MEK1 (Ser218/222)/ H M R WB Pur Rb IgG 200 µg 07-461
MEK2 (Ser222/226)
Anti-MEK1 H M R IP IPK WB Pur Rb IgG 200 µg 07-641