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220-967-4
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Showing 1-30 of 65 results for "220-967-4" within Technical Documents
Product Information Sheet - 11167103001
1 �g pBR322 DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - 10822221001
1 �g pBR322 DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - 11004816001
1 �g pBR322-DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
Product Information Sheet - 10827754001
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
Product Information Sheet - KPNIRO
Eco RI fragments are ligated with 1 U T4-DNA
ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol
Product Information Sheet - 11117807001
pUC18
4 33 0 1 0 0 0 0
0717.111215610019
Ligation and
recutting assay
Ksp I fragments obtained by complete digestion of 1 �g
�DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 481 220)
Product Information Sheet - 10862495001
digestion of 1
�g �DNA are ligated with 1 U T4-DNA ligase (Cat. No.
10 481 220 001) in a volume of 10 �l by incubation for
16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2, 5
Product Information Sheet - HPAIRO
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2
Product Information Sheet - 10656305001
001
SuRE/Cut Buffer B Restriction enzyme
incubation
5 × 1 ml (10� conc.
solution)
11 417 967 001
SuRE/Cut Buffer H Restriction enzyme
incubation
5 × 1 ml (10� conc.
solution)
11
Product Information Sheet - HINFIRO
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
Product Information Sheet - 10775207001
µg pBR322 DNA are ligated with 0.1 units T4-DNA
ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 1 mM dithioerythritol, 1
Product Information Sheet - 11198939001
digestion of
1 �g λDNA are ligated with 1 U T4-DNA ligase (Cat.
No. 481 220) in a volume of 10 �l by incubation for 16 h
at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM dithioth-
reitol, 1
Product Information Sheet - 11168860001
of
1 �g �DNA are ligated with 1 unit T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �I by incu-
bation for 16 h at 4°C in 66 mM Tris-HCI, 5 mM MgCI2,
5 mM Dithioerythritol
Product Information Sheet - 10835307001
1 �g pBR322 × DNA are ligated with 1 U T4 DNA
Ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM
MgCl2, 5
Product Information Sheet - ECORVRO
001
SuRE/Cut Buffer B Restriction enzyme
incubation
5 × 1 ml (10� conc.
solution)
11 417 967 001
SuRE/Cut Buffer H Restriction enzyme
incubation
5 × 1 ml (10� conc.
solution)
11 417
Product Information Sheet - CLAIRO
digestion of 1�g
� DNA are ligated with 1 U T4 DNA Ligase (Cat. No.
10 481 220 001) in a volume of 10 �l by incubation for
16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5
Product Information Sheet - 11464825001
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - 10776777001
1 �g pBR322 DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol
Product Information Sheet - 11102982001
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
Product Information Sheet - ECORIRO
digestion of
1 �g �DNA are ligated with 1 U T4 DNA Ligase (Cat.
No. 10 481 220 001) in a volume of 10 �l by incubation
for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2
Product Information Sheet - RSAIRO
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - BSTXIRO
digestion of
1 �g DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM
Product Information Sheet - PVUIIRO
digestion of
1 �g �DNA are ligated with 1 U T4 DNA Ligase (Cat.
No. 10 481 220 001) in a volume of 10 �l by incubation
for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5
Product Information Sheet - NSIIRO
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - 11288075001
digestion of
1 �g � DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4°C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - 10775266001
001
SuRE/Cut Buffer B Restriction enzyme
incubation.
5 × 1 ml (10� conc.
solution)
11 417 967 001
SuRE/Cut Buffer H Restriction enzyme
incubation.
5 × 1 ml (10� conc.
solution)
11 417
Product Information Sheet - MLUIRO
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 481 220) in a volume of 10 �l by incubation
for 16 h at 4° C in 66 mM Tris-HCl, 5 mM MgCl2,
5 mM Dithiothreitol
Product Information Sheet - 11441337001
digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No. 10 481 220 001) in a volume of 10 �l by incu-
bation for 16 h at 4 °C in 66 mM Tris-HCl, 5 mM MgCl2,
5
Product Information Sheet - SALIRO
1 �g pBR322 DNA are ligated with 1 unit T4-DNA
ligase (Cat. No. 10 481 220 001) in a volume of 10 �l by
incubation for 16 h at 4°C in 66 mM Tris-HCl,
5 mM MgCl2, 5 mM
Product Information Sheet - 11243497001
and 4 h
incubation time are recommended.
Ligation and
recutting assay
Sfu I fragments obtained by complete digestion of
1 �g �DNA are ligated with 1 U T4-DNA ligase
(Cat. No.10 481 220 001)
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