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T7 RNA Polymerase - recombinant, expressed in E. coli
T7 RNA Polymerase is a DNA-dependant RNA Polymerase that exhibits a very high specificity for the T7 promoter sequence. The polymerase is useful for synthesizing large amounts of RNA suitable for in vitro translation and anti-sense RNA research.
Analytical Method: Copper (total) in effluents, high organic content
Photometric determination with Cuprizon subsequent to acid mineralisation
Optimize Sample Performance with the SMC® Plate Washer Evaluation Kit
Learn how to optimize your plate washer performance with the SMC® Plate Washer Evaluation Kit and assay precious samples with confidence.
FAMLS Online Plate Map
FAMLS compounds are conveniently provided at 5µg per well, enough for multiple injections. These are high purity (>95%) compounds supplied in an economical, ready to use format. Compounds are arranged in a 96-well polypropylene plate.
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.
Calcitonin Gene-Related Peptides
Sigma-Aldrich offers many products related to calcitonin gene-related peptides for your research needs.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Measurement of acetic acid vapor in the workplace using passive sampling SPME and on-fiber derivatization with 1-pyrenyldiazomethane: Comparison with active sampling
Measurement of acetic acid vapor in the workplace using passive sampling SPME and on-fiber derivatization with 1-pyrenyldiazomethane: Comparison with active sampling.
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
Efficient Analysis of Kava Extract: New phytochemical certified reference material mixes for ginger and kava
Learn more about our Kava CRM solution for use in quantitative and qualitative analysis of kavalactones and flavokavains in kava products to confirm their potency and authenticity.
Protocols for the Fmoc SPPS of Cysteine-containing Peptides
Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.
Gelling Agents – Plant Tissue Culture Protocol
Find instructions for preparing alginate, agargel, and phytagel agar used to solidify media for plant tissue culture. Also provided are criteria for selecting the appropriate product for your application.
Absolute Quantification of Serum Proteome
Absolute Quantification (Protein-AQUA™™) is a targeted quantitative proteomics technique that exhibits robust efficacy and is being increasingly utilized for a wide variety of quantitative proteomics studies.
3D Organoid Culture: New In Vitro Models of Development and Disease
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Affinity Purification with Ultrafree®-MC Centrifugal Filters
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
Poly(2-Oxazoline)s: The Versatile Polymer Platform for Biomedicine
The introduction of polymers into the biomedical field has opened new avenues in tissue engineering, implant design, biosensing, and drug delivery.
Selecting Orthogonal Building Blocks
Novabiochem® product range has one of the largest collections of orthogonally and quasi-orthogonally protected tri-functional amino acids. These derivatives are useful tools for the synthesis of cyclic and branched peptides and peptides carrying side-chain modifications.
Integration of Sigma® TransPlex® WTA and Complete WTA2 Kits with the Agilent Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated
into existing Agilent workflows
Periodic Table of the Elements
Download the Periodic Table of the Elements with names, atomic mass and number in printable .pdf and image format. Use the periodic table chart by element names in alphabetical order for quick research, reference and lab use.
Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Desalting Samples Using Mini Dialysis Kit
Mini Dialysis Kit from Cytiva is designed for the dialysis of small sample volumes with minimal handling and sample loss and reduces the pronounced streaking on 2-D gels caused by low-molecular-weight contaminants.
Recent Trends in Self-Healing Soft Electronic Materials and Devices
Dr. Tan and researcher introduce recent trends in Self-healing Soft Electronic Materials and Devices. The emergence of smart, functional SHPs will be highly beneficial to the advancement of the next-generation self-healing soft electronic devices. Autonomously self-healing devices could help to
NF-κB and Inflammation
Chronic inflammation is an underlying factor in the development and progression of many of the chronic diseases of aging, such as arthritis, atherosclerosis, diabetes, and cancer.
Extractables Study with LC-UV/MS
Reliable identification and quantification of unknown extractables of a single-use system (SUS) with a 21 component CRM mix for liquid chromatography (LC-UV/MS) methods using Ascentis® C18 column.
Poly(2-oxazoline)s (POx) in Biomedical Applications
Poly(2-oxazoline)s (POx) can be viewed as conformational isomers of polypeptides. They are synthesized via living cationic ringopening polymerization (LCROP) of 2-oxazolines and were almost simultaneously discovered in 1966 by the groups of Litt, Tomalia, Fukui and Seeliger.
Immunohistochemistry Applications in Gastrointestinal Cancer
Colorectal cancer is the third most common cancer in both men and women. An estimated 136,000 cases of colorectal cancer are expected to occur in 2016.
Coupling Small Ligands Through Carboxyl Groups via a Spacer Arm
Ligands are coupled in a simple one-step procedure in the presence of a coupling reagent, carbodiimide. The carbodiimides may be regarded as anhydrides of urea.
What Are Biomaterials?
Biocompatible / Biodegradable Polymer Tutorial
Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
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