, eds., Meth.
Enzymology, 59, 260 (1979).
5. Boussif, O. et al, Proc. Nat. Acad. Sci., 92, 7297
(1995).
6. Bruns, R.F. et al., Anal. Biochem., 132, 74 (1983).
7. Supplier Data
Use of PEI
glycosides as substrates and ligands
of carbohydrases. Meth. Enzymol., 160(pt. A), 45-
59 (1988).
HLD/RXR 7/03
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc
concentration of approximately
100 µg/mL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl,
0.25 mM DTT, 0.1 mM EGTA and 30% glycerol.
Purity: ≥ 75% (SDS-PAGE)
Molecular weight: ~59 kDa
Specific Activity: ≥ 10 units
Preparation
Suspend 59 g in 1 litre of demin. water, add 4.6 ml XLT4 Agar
Supplement solution and heat the medium in a boiling water-
batch (not on a heating-plate!). Cool to approx. 50 °C and pour
plates
amino acid mitochondrial uncoupling protein (1). It is only 59%
homologous with UCP1 found in brown adipose tissues. Mouse UCP2 (chromosome 7)
has approximately 95% homology with human UCP2 located on
testosterone antibody binding site by stepwise
optimisation of the CDRs. Immunotechnol., 4, 59-
69 (1998).
7. Zhou, E.M. et al., Development and evaluation of
an IgM-capture ELISA for detection of recent
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Precautions
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
NM 004935. It
is supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM
NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: CDK5 ∼59 kDa
p25 ∼49 kDa
Purity: ≥70%
Technique
for Typing Red Blood Cells With a Positive Direct
Antiglobulin Test. Transfusion, 22(1), 59-61
(1982).
7. Krajewski, W.A., Effect of In Vivo Histone
Hyperacetylation on the State of Chromatin Fibers
(MM:SS) - 00:00 to 59:59
• Stir(rpm) – 100 to 2000
• Fan Control – Manual / Auto
• Manual Fan (rpm) – 2800 to 6800*
• Auto Fan/Temperature Control (Temp (C)