600-22-6

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10% PEG 600 19. 28531 0.1 M TRIS-HCl pH 8.0, 20% PEG 600 20. 11075 0.1 M TRIS-HCl pH

cells for 5 minutes at 600 × g. Note: For cells in suspension, perform Step 2.3 only (the Centrifugation Step). MITOISO2pis Rev 09/22

Product Information Sheet - NA9604

plate. Turn off vacuum. 16. Add 600 µL of Wash Solution 1 and apply vacuum until all the solution passes through the binding plate. Turn off vacuum. 17. Add 600 µL of Wash Solution 2 and apply vacuum

Product Information Sheet - MITOISO3

the supernatant. 6. Resuspend the cell pellet in 1 ml of 1× Buffer B. 7. Add 10 µL of the resuspended cell sample to 990 µL of ultrapure water and read the OD at

Product Information Sheet - CE0050

Lysis and Separation Working Solution fresh daily, by adding 6 µL of P8340 to 600 µL of L2417. CE0050pis Rev 07/22

Product Information Sheet - MAK127

Table 2. 600 µL of the Master Reaction Mix is required for each reaction. Table 2. Master Reaction Mix Reagent Volume Reagent B 20 µL Reagent C 600

Product Information Sheet -RAB0166

Product Information Sheet - H7787

Place spin column back in this same collection tube. 6. Load the prepared cell extract on the column. The column will hold up to 600 µL of extract at one time. 7. Centrifuge

Product Information Sheet - MAK487

cleared by mixing 600 µL milk with 100 µL 6 N HCl. 2. Centrifuge for 5 minutes at 14,000 × g. 3. Transfer 300 µL supernatant into a clean tube and neutralize with 50 µL

Absolute Quantifi cation of the Lower Abundance Proteome Through Immunoaffi nity Depletion of the Twenty Most Abundant Proteins in Human Serum

3 +1 390.2 b 6 +1 687.4 b 7 +1 786.3 y 7 +2 407.3 y 6 +2 343.1 0 50 100 150 200 250 300 350 400 450 500 550 600 650

Data Sheet - C8241

C8241dat Rev 03/22 1

Product Information Sheet - MITOISO1

to remove lipids, which may be present in the tissue. 6. Transfer the homogenate to a 2 mL Eppendorf tube. Centrifuge the sample at 600 × g for 5 minutes. 7. Carefully transfer the

Product Information Sheet - H0413

unbound protein from the filter plate using 600 µL of Wash Buffer with centrifugation. Empty the collection plate. 9. Repeat the wash step (Step 8) with 600 µL of Wash Buffer. 10.

FlowCellect™ Mouse Viable Treg Characterization Kit

in the dark for 15 minutes. 6. Spin down cells at 600 x g for 3 minutes and discard buffer. 7. Resuspend cells in 1 mL of 1X Wash Buffer and spin down cells at 600 x g

Userguide -MP0050

600 µL 300 µL Add 2 µL Sample Add 2 µL Sample Add 2 µL Positive Control DNA Add 2 µL Fresh Cell Culture Medium

Product Information - H2250

540 8.91 HCl-Acetone3 640 4.68 HCl-Acetone3 545 5.01 Acetone3 388 63.10 pH 9.9/11.73 600 3.98 pH 9.9/11.73 STABILITY / STORAGE AS SUPPLIED: Store powder at 2-8°C. ProductInformation

Instruction Manual for EZ-Zyme Chromatin Prep Kit

tube tightly and place in a 400-600 mL glass beaker filled with approximately 200 mL of water. 5. Microwave beaker with tube until water in beaker begins boiling. 6. Remove beaker and vortex tube

Data Sheet - C3905

mM Tris-HCl (pH 7.5), 10 mM MgCl2, 22 mM NH4Cl, 10 mM β-mercaptoethanol, and 5% glyercol.6 Elution of protein may be affected by adding NaCl from 100 mM up to 600 mM to this buffer.3 Removing tightly

Manual Methods for color measurements

, accurate to 0.001 grams (g) 5 , confirm with 6 , and click on 7 to switch to the measurement procedure. 7 5 6 22

RABBIT ANTI-RAD51 AFFINITY PURIFIED POLYCLONAL ANTIBODY

5. Let air dry 6. PBS 5min 7. Incubate in 50mM ammonium chloride 30 min 8. PBS 5min 9. Blocking serum 30-45min 10. Primary antibody 90min - dilution 1:100 -

S.D.S. Purified Water Storage and Distribution System

NETHERLANDS Tel. 076-5022000 Fax 076-5022436 NORWAY Tel. 22 67 82 53 Fax 22 66 04 60 POLAND Tel. 22-669 12 25 22-663 70 31 Fax 22-663 70 33 PUERTO RICO Tel. (787) 273-8495 Fax (787) 747-6553

Product Information Sheet - MAK505

by mixing 600 µL milk with 100 µL of 6 N HCl. 2. Centrifuge mixture for 5 minutes at 14,000 rpm. 3. Transfer 300 µL supernatant into a clean tube and neutralize

Product Information Sheet - SE120143

temperature, with shaking (600 rpm). 5. Remove liquid from all wells. Wash wells three times with 300 µL of 1x wash buffer (see Reagent Preparation). Blot on absorbent paper towels. 6. Add 100 µL of anti-alpha

DataSheet - G8132

hydrolysis of conjugated steroid metabolites to be a one-hour incubation at 55 °C at pH 5.2, using 600 units of enzyme per mL of urine.4 β-Glucuronidase Type L-II from keyhole limpet is a crude

SecureSeal References

alcohol) Solutions Studied by Fluorescence Correlation Spectroscopy. Biomacromolecules. 8: 1595-600. Ng SB, Turner EH, Robertson PD, Flygare SD, Bigham AW, Lee C, Shaffer T, Wong M, Bhattacharjee

Product Information Sheet-udghl-ro

of analysis, see section, Contact and Support. 1 2 3 4 5 6 https://www.sigmaaldrich.com 06 22 .1 17 84 45 50 01 14

Product information sheet - XNAS2

Product Information Sheet-XNAS2

Product Information Sheet - CS0006

Digest Enzyme CS0006C 1 mL Red cap/ vial 30× Probe CS0006D 600 µL Brown vial 10× Development Solution CS0006E 600 µL Brown vial Component Information Assay Buffer (Component

Product Information Sheet - LI0601BC

activity. See Asay Procedure, Step 6. Mix 60 µL of the substrate and 60 µL of the Chromogen for each well used. Example: for 10 wells, mix 600 µL of substrate and 600 µL of Chromogen.

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