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STED Sample Preparation Guide
Sigma-Aldrich 76085 (mouse)
77671 (rabbit)
Alexa Fluor 633 633 775 life technologies A-21063 (mouse)
A-21070 (rabbit)
ATTO 633 633 775 Sigma-Aldrich 78102 (mouse)
41176 (rabbit)
Abberior
DataSheet - P3752
, K. et al., Br. J. Nutr., 74(1), 3-17 (1997).
8. López, R.S. et al., Acta Agriculturae Scandinavica,
Section B — Soil & Plant Science, 57(1), 65-73
(2007).
9. Smith, J.R. et al., Physiol.
Product Information Sheet-11585649910
Cat. No.
Reagents, kits
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
CDP-Star, ready-to-use 2 x 50 ml 12 041 677 001
NBT/BCIP Stock Solution 8 ml 11 681 451 001
1 2 3
1 2 3
Product Information Sheet - 11585622910
100 �g 11 426 320 001
Biotin-High Prime 100 �l 11 585 649 910
CSPD, ready-to-use 2 × 50 ml 11 755 633 001
CDP-Star, ready-to-use 2 × 50 m 12 041 677 001
DIG-High Prime 160 �l 11 585 606 910
High Prime
Product Information Sheet-digutp-ro
≥ 20 U/μl 10 881 767 001
5,000 U, ≥ 20 U/μl 10 881 775 001
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
DIG-labeled Control RNA 50 μl, 100 μg/ml DIG-labeled RNA 11 585 746 910
CDP-Star, ready-to-use
XP725 Antibody Number-Lot Number 071M4826
75 76
1.2 5 6 7 8 29 30 31 32 53 54 55 56 77 78 79 80
1.3 9 10 11 12 33 34 35 36 57 58 59 60 81 82 83 84
1.4 13 14 15 16 37 38
Data Sheet - E6779
508-519 (1991).
5. Field, J. et al., Mol. Cell. Biol., 8(5), 2159-2165
(1988).
6. Antebi, A., and Fink, G.R., Mol. Biol. Cell, 3(6),
633-654 (1992).
7. Blackstone, Hope E., “Nanog modification
Product Information Sheet - 11526537910
11 096 176 001
CDP-Star, ready -to-use 2× 50 ml 12 041 677 001
CSPD , ready-to-use 2× 50 ml 11 755 633 001
DIG Easy Hyb (ready-to-use
hybridization solution without
formamide)
500 ml 11 603 558 001
Product Information Sheet - 11526529910
11 096 176 001
CDP-Star, ready -to-use 2× 50 ml 12 041 677 001
CSPD , ready-to-use 2× 50 ml 11 755 633 001
DIG Easy Hyb (ready-to-use
hybridization solution without
formamide)
500 ml 11 603 558 001
Product Information Sheet - 11373099910
11 096 176 001
CDP-Star, ready -to-use 2× 50 ml 12 041 677 001
CSPD , ready-to-use 2× 50 ml 11 755 633 001
DIG Easy Hyb (ready-to-use
hybridization solution without
formamide)
500 ml 11 603 558 001
Product Information Sheet - PSTIRO
certificate of
analysis.
References 1 Brown, N. L. & Smith, M. (1976) FEBS Lett. 65, 284.
2 Malyguine, E. et al. (1980) Gene 8, 163.
3 Kessler, C. & Manta,V. (1990) Gene 92, 1–248.
4 Rebase The Restriction
Product Information Sheet-11755633001
.
y Version: 08
Content Version: June 2021
Cat. No. 11 755 633 001 2 x 50 ml
Store the product at +2 to +8°C.
https://www.sigmaaldrich.com
Product Information Sheet-11585606910
inches, 18 x 24 cm, Not
available in US
11 666 916 001
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
DIG-labeled Control DNA 50 μl, 5 μg/ml DIG-labeled plasmid DNA 11 585 738 910
DIG-High Prime DNA
BioFiles-Issue
610 605 646 70 150000 HeNe laser Alexa 610
Atto 620 620 641 50 120000 HeNe laser
Atto 633 629 657 64 130000 HeNe laser, Ruby laser Alexa 635
Atto 635
Application Note - Step-by-Step Workflow for Small Sample Quantitative Real-Time PCR Confirmation of Microarray Results
Master 04 887 301 001 10 x 500 reactions, 20 µl volume
Universal ProbeLibrary Set, Human 04 683 633 001 1 Set
Universal ProbeLibrary Set, Human Reference Gene Assays 05 046 114 001 1 Set
HIGHPURE
Product Information Sheet - G4666
8. Transfer the tube to a heat-block at 65 °C and incubate for 3 minutes.
9. Add 700 µL of acidic phenol (pre-warmed to 65°C), vortex vigorously to mix and incubate for 3
minutes, at 65°C.
Panorama M. tuberculosis Gene Arrays
8. Transfer the tube to a heat-block at 65 °C and incubate for 3 minutes.
9. Add 700 µL of acidic phenol (pre-warmed to 65°C), vortex vigorously to mix and incubate for 3
minutes, at 65°C.
Product Information Sheet-11175033910
Ordering Information
Product Pack Size Cat. No.
Reagents, kits
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
DIG Easy Hyb Granules 6 bottles, Granules for 6 x 100 ml 11 796 895 001
High Pure PCR Product Purification
Product Information Sheet-11699075001
ready-to-use 2 x 50 ml 11 755 633 001
DIG Easy Hyb 500 ml 11 603 558 001
Blocking Reagent custom fill 10 057 177 103
Lumi-Film Chemiluminescent Detection
Film
100 films, 8 x 10 inches, 20.3 x 25.4 cm
Application Note - Relative Quantification of Multiple mRNA Targets and Reference Genes in Spinocerebellar Ataxia
8 µl/well + 2 µl primer probe mix
Primer-probe mix for target assays: Primer-probe mix for reference assays:
15 µl 5 µM forward primer
15 µl 5 µM reverse primer
5 µl UPL probe
65
Product Information Sheet-11175025910
Anti-Digoxigenin-AP, Fab fragments 150 U, 200 μl 11 093 274 910
CSPD, ready-to-use 2 x 50 ml 11 755 633 001
Lumi-Film Chemiluminescent Detection Film 100 films, 7.1 x 9.4 inches, 18 x 24 cm,
Not available
Product Information Sheet-03353583910
buffer under constant
stirring on a heating block (+65°C), or
heat in a microwave oven and autoclave.
The solution remains opaque.
Store at +2 to +8°C
initially; after first
usage, store in aliquots
Mouse STEMCCA Cre-Excisable Constitutive Polycistronic (OKS) Lentivirus Reprogramming Kit
pluripotent stem cells from mouse embryonic
and adult fibroblast cultures by defined factors. Cell 126: 633-676.
2. Okita ,et al. (2007) Generation of germline-competent induced pluripotent stem cells. Nature
Mouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit
pluripotent stem cells from mouse embryonic
and adult fibroblast cultures by defined factors. Cell 126: 633-676.
2. Okita ,et al. (2007) Generation of germline-competent induced pluripotent stem cells. Nature
XP725 Antibody List Lot 129K4831
M NP_001460.1 y n/d n/d
290 Glutamic Acid Decarboxylase 65 (GAD 65) G4913 2572 GAD2 P NP_000809.1 y y y
291 Glutamic Acid Decarboxylase 65 (GAD 65) G5038 2572 GAD2 P NP_000809.1 y y y
292 Glutamic Acid
XP725 Antibody List Lot 129K4830
M NP_001460.1 y n/d n/d
290 Glutamic Acid Decarboxylase 65 (GAD 65) G4913 2572 GAD2 P NP_000809.1 y y y
291 Glutamic Acid Decarboxylase 65 (GAD 65) G5038 2572 GAD2 P NP_000809.1 y y y
292 Glutamic Acid
XP725 Antibody List Lot 089K4791
M NP_001460.1 y n/d n/d
290 Glutamic Acid Decarboxylase 65 (GAD 65) G4913 2572 GAD2 P NP_000809.1 y y y
291 Glutamic Acid Decarboxylase 65 (GAD 65) G5038 2572 GAD2 P NP_000809.1 y y y
292 Glutamic Acid
List of Antibodies
Methyltransferase G6919 P y n/d n/d
290 Glutamic Acid Decarboxylase 65 (GAD 65) G4913 P y y y
291 Glutamic Acid Decarboxylase 65 (GAD 65) G5038 P y y y
292 Glutamic Acid Decarboxylase (GAD65/67) G5163 P
XP725 Worksheet for Lot Number 071M4826
BiP G8918
629 4 2 1 5 GRP94 G4420
630 4 2 1 6 GRP94 G4420
631 4 2 1 7 hABH1 A8103
632 4 2 1 8 hABH1 A8103
633 4 2 2 1 hABH2 A8228
634 4 2 2 2 hABH2 A8228
635 4 2
Bioreactor Systems for Bone Tissue Engineering
of
cadherins, which are critical for BMP-2-induced osteogenic
differentiation. J Bone Miner Res 13, 633, 1998.
53. van den Dolder, J., Spauwen, P.H., and Jansen, J.A.
Evaluation of various seeding techniques
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