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Column Packing and Preparation for Size Exclusion Chromatography

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Multiplexed Evaluation of Soluble Immune-Checkpoint Molecules | MILLIPLEX® Assays

Research evaluating immune checkpoints may help identify those likely to benefit from PD-1/PD-L1 immunotherapy and reveal new immunotherapy targets for future investigation. See how the MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 was used to multiplex immune checkpoint molecules for

PYBOX Ligand - Large Binding Site & Rigid Scaffold

The PYBOX ligand, consisting of a pyridine ring flanked by two oxazoline groups, was introduced in 1989 by Nishiyama.

Carreira DOLEFIN Ligands

The selective preparation of diarylmethine stereogenic centers is a challenging endeavor in chemical synthesis, especially when there is little differentiation between the two aryl groups

Synthesis and HILIC/MS Analysis of Acylcarnitines

HILIC mode chromatography using an Ascentis® Express OH5 resolved the majority of the acyl-L-carnitines tested. It may be possible to use MS/MS and particular fragment ions to resolve the two coeluting pairs.

Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge

Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.

DuPhos and BPE Ligands

Asymmetric hydrogenation reactions represent the ideal process for the commercial manufacture of single-enantiomer compounds, because of the ease by which these robust procedures can be scaled up and because of the low levels of byproducts generated in these asymmetric hydrogenations.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Metal Borohydrides as Hydrogen Storage Materials

An article about metal borohydrides as hydrogen storage materials

Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli

In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Single-Double Multi-walled Carbon Nanotubes

Find unique properties & applications of single (SWNTs) , double (DWNTs) & multi walled carbon nanotubes (MWCNTs).

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

Protocols for the Fmoc SPPS of Cysteine-containing Peptides

Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.

Solubility Table of Compounds in Water at Temperature

Check the solubility of 128 common inorganic compounds (salts and acids) in water at different temperatures from 0 degrees Celsius to 100 C. Compound formulas are also given in this table of solubility in relation to temperature.

Detection of Campylobacter spp. and Enterobacter Sakazakii in Food with a New Test Kit System

Detection of Campylobacter spp. and Enterobacter sakazakii in food with a new test kit system.

Puriﬁcation using StrepTrap™ HP 1 mL and 5 mL

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Barrier Formation and Permeability Assays using Millicell^® Hanging Cell Culture Inserts

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

Organic Semiconductor Laser Materials

Immunohistochemistry: Current Applications in Skin Cancer

Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.

Detection of Alzheimer’s Disease Biomarkers | MILLIPLEX^® Assays

Learn how multiplex detection of Alzheimer’s disease biomarkers, using the MILLIPLEX®Human Amyloid Beta and Tau Magnetic Bead Panel, allows for a different perspective on neurodegenerative disease research.

Poly(2-oxazoline)s (POx) in Biomedical Applications

Poly(2-oxazoline)s (POx) can be viewed as conformational isomers of polypeptides. They are synthesized via living cationic ringopening polymerization (LCROP) of 2-oxazolines and were almost simultaneously discovered in 1966 by the groups of Litt, Tomalia, Fukui and Seeliger.

Poly(2-Oxazoline)s: The Versatile Polymer Platform for Biomedicine

The introduction of polymers into the biomedical field has opened new avenues in tissue engineering, implant design, biosensing, and drug delivery.

Perfluorosulfonic Acid Membranes for Fuel Cell and Electrolyser Applications

Advances in the electrochemical conversion of water to and from hydrogen and oxygen have principally been achieved through the development of new materials and by understanding the mechanisms of the degradation of proton exchange membrane fuel cells (PEMFC) during operation.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

Immunohistochemistry Applications in Gastrointestinal Cancer

Colorectal cancer is the third most common cancer in both men and women. An estimated 136,000 cases of colorectal cancer are expected to occur in 2016.

Organic Photovoltaics in IoT, Architecture, and Wearables

Advances in OPV materials & device design; OPV technology as an energy source, enabling larger deployment of low-power, low-maintenance, & environmentally friendly hardware solutions is discussed.

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