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Cytology and microscopy
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Detergent-Free Nuclear Protein Extraction
Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.
Subculture of Adherent Cell Lines
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
Trypsin Cell Dissociation Protocol
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Enzymatic Hydroxyproline Assay Protocol
Assay protocol for the colorimetric detection of Hydroxyproline in biological samples using the Hydroxypoline assay kit.