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Deoxyribonuclease I
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Long and Accurate PCR Amplification of DNA (D8045)
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Extract-N-Amp™ FFPE Tissue PCR Protocol
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
Multiplex Real-Time PCR
Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.
Expand™ Long Template PCR System Protocol Troubleshooting
This polymerase mixture produces a high yield of PCR product from genomic DNA.
RNA Handling When Using the First Strand cDNA Synthesis Kit for RT-PCR (AMV)
First Strand cDNA Synthesis Kit for RT-PCR (AMV).
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
DNA Amplification using Illustra PuReTaq Ready-To-Go PCR Beads
This page shows DNA amplification using illustra PuReTaq ready-to-go PCR beads from Cytiva.
End Point PCR Protocol for Long and Accurate DNA Amplification
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Integration of Sigma® TransPlex® with Agilent Microarray
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
SeqPlex DNA Amplification Kit Protocol
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
Plant-Based PCR Inhibitors & Master Mix Performance
Evaluate PCR inhibitors and enzyme selection using GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix, and competitors.
Recombinant Selection
Various DNA manipulation techniques from minipreps to gel electrophoresis are discussed for molecular biology experiments.
WGA Using GenomiPhi™
GenomiPhi™ method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.
DNA Extraction & WGA Amplification from Soil Samples
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Saliva DNA Extraction & WGA Amplification
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Whole Genome Amplification: Blood Card Protocol
Efficient extraction of genomic DNA from blood cards for whole genome amplification and analysis.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Simple DNA isolation protocol from whole blood samples for genomic analysis, followed by GenomePlex® amplification.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
REDExtract-N-Amp™ Plant PCR Kit Protocol
REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.
Optimized PCR-based Detection of Mycoplasma
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
ABI3900 Synthesizer Columns with CUTAG CPG Frits
Next generation CPG Frit support for reliable, cost-effective oligo synthesis from Proligo®, for ABI 3900 and MerMade synthesizers
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