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Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns

The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Ion Pairing for Analysis of Phosphonate Compounds

Phosphonates are a class of compounds commonly used for therapeutic applications of antiviral drugs. These compounds can be difficult to analyze due to their highly polar nature and lack of UV active chromophores.

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.

Cyclodextrins

Many metabolically important compounds, such as lipid-soluble vitamins and hormones, have very low solubilities in aqueous solutions. Various techniques have been used to solubilize these compounds in tissue culture, cell culture, or other water-based applications.

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Cell Freezing and Banking

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Oxidative Stress in Cell Culture

Read how oxygen, oxygen free radicals, oxidative stress and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing of monoclonal antibodies

Transferrin in Cell Culture

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

Counting of Cells

Trypan blue method of cell counting is inexpensive and readily available method. The method is based on principle that living cells exclude trypan blue dye and appear bright, while dead cells take up dye and appear blue. Watch video to

Modified Amino Acids

Modified amino acids are inhouse manufactured amino acid derivatives with specific properties enabling the intensification of cell culture processes.

Angiogenesis Assays

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

3D Cell Culture with Corning Microplates

Take a deep dive into the world of 3D cell culture courtesy of Corning and understand why the Corning® spheroid microplate can be an asset in the evolution of 3D cell culture.

Hepatotoxicity/Cytotoxicity Assays

The hepatotoxicity/cytotoxicity assays provide a means to measure cell death due to the cytotoxic effect of a test compound. We provide hepatotoxicity/cytotoxicity assays for all small molecule formulations such as pharmaceuticals, industrial chemicals and consumer products.

MISSION™ shRNA Library: Next Generation RNA Interference

Work and refinement of the RNAi technology has exploded in recent years. We now know the basic mechanism of the endogenous RNAi pathway, that the pathway is present in most eukaryotes,3 and how cellular machinery can be harnessed to silence

Resolving by Mass

GC Injection Port Issues: Two Case Studies (Peak Tailing and Low Response)

Proper product selection and preventative maintenance for the GC inlet are vital elements that impact performance of the GC instrument.

Melamine Analysis via GC-MS

Sigma-Aldrich presents an article about the analysis of melamine and related compounds in dog food with the use of more economical gas chromatography-mass spectrometry (GC-MS) instrumentation.

Analysis of Remdesivir and other Antiviral Drugs Using LC-MS/MS

We present a fast and robust HPLC-MS/MS method that can be used for Remdesivir and three structural antiviral analogues

Separation of Alcohols and Esters in Spirits using Serially Connected GC Columns

The Best Approaches to Gas Purification

The Derivatization and Analysis of Amino Acids by GC-MS

Proline Derivatization and Enantioresolution by Chiral GC

Amino acids are building blocks of peptides and proteins. Enantiomeric separation of these molecules can be performed through chromatography by chiral stationary phases. This article describes the chiral GC analysis of one amino acid, proline, after achiral derivatization.

Ultra-Pure MALDI Matrices

One of the most important aspects of our ultra-pure MALDI matrix substances is their ability to dissolve rapidly and completely; a brief vortex mixing is typically sufficient.

GC Injection Port Septa: Use, Maintenance, Storage and Handling

Proper handling and preventative maintenance instructions for injection port septa; tips to improve the performance of gas chromatography instruments.

Measuring Flows For Gas Chromatography Volumetric vs. Mass

Solid Phase Extraction (SPE) Overview

Solid phase extraction is a form of digital (step-wise) chromatography designed to extract, partition, and/or adsorb one or more components from a liquid phase (sample) onto stationary phase (sorbent or resin).

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

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