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Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

Enzyme Commission Numbers

Complete List of Enzyme Commission Numbers for metabolics research.

Alkaline Phosphatase

We offer a broad range of alkaline phosphatase enzymes and substrates that are optimized for conjugation to antibodies and other proteins for your specific application needs.

Tubulin

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Nanodisc Technology: A Revolutionary System for Study of Membrane Proteins

Read our article about how the Nanodisc system allows for structural studies of membrane proteins.

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

VEGF Pathway

Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

This article shows a comparison of the quantitative viability bioassays MTT, alamarBlue™, and Guava® ViaCount®.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

ThermaStop™ PCR Additive

PCR additive added to master mix that stops nonspecific bands after cool down.

Intermetallic Hydrides with High Dissociation Pressure

Self-Assembly in Organic Thin Film Transistors for Flexible Electronic Devices

Flexible electronic circuits and displays based on organic active materials are future generations of products that may eventually enter mainstream electronics market.

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

Automated IgG Purification Using PureProteom

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

Detect, Visualize and Quantify Single Post-Translational Modifications

Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications

Single Molecule Detection with Atto 647N NTA

Significant improvement of single molecule tracking by using superior fluorescent NTA-Atto 647N conjugate

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

Optimizing Crude Sample Plant PCR

Overcoming potent inhibitors and sample diversity in direct plant PCR.

Safer High-performance Electrodes, Solid Electrolytes, and Interface Reactions for Lithium-ion Batteries

Li-ion batteries are currently the focus of numerous research efforts with applications designed to reduce carbon-based emissions and improve energy storage capabilities.

Single-Walled Carbon Nanotubes synthesized by the Super-Growth Method: Properties & Applications

Single-Walled Carbon Nanotubes synthesized by the Super-Growth Method & their properties & applications, including dispersing SGCNTs, SGCNT-polymer composites & SGCNT-metal composites are discussed.

Drug Conjugate Analysis using β-Glucuronidases

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

Organic Semiconductor Laser Materials

Graphene Field Effect Transistors for Biological and Chemical Sensors

Smart Nanofiber Meshes as a Local Drug Delivery Platform

Professor Mitsuhiro Ebara provides insights on several types of smart nanofiber mesh systems that have been explored for different drug delivery purposes.

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