Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
The GenomiPhi™ method of WGA from Cytiva uses Phi29 DNA polymerase, a highly processive enzyme with excellent strand-displacement activity, in combination with random-sequence hexamer primers (random hexamers) to amplify DNA in an isothermal process—a thermal cycler is not required.
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated
into existing Agilent workflows
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the
Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
Blue White Screening; DNA Minipreps; Screening by Restriction Digestion; Screening by PCR; Confirm cut plasmid sizes by agarose gel electrophoresis; DNA Maxipreps; DNA Precipitation; RNase Treatment; Clean-up of DNA
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.