Advanced gene editing

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Zinc Finger Nuclease References: Human, Rat, Mouse, Zebrafish, Plasmodium, Xenopus, Rainbow Trout, Mosquito, Pig, Rabbit, Cattle, CHO, ZFN Technology, Reviews, Posters, Label License

Small RNA and miRNA (microRNA) Sequencing

A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.

Safe Lentivirus Handling

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.

Nuclease-Free Water at Your Fingertips

An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.

TRC Design and pLKO.1 Vector FAQs

The MISSION® shRNA clones, designed by the TRC, are pre-cloned into the pLKO.1-Puro vector. This lentiviral vector allows for propagation in bacterial culture and selection of inserts in mammalian cells.

Small Molecule CRISPR Enhancers

Modulation of homology-directed repair (HDR) within the context of CRISPR-genome editing has led to the identification of small molecules that enhance CRISPR-mediated HDR efficiency in various cell types.

Methods for Enhancing Lentiviral Transduction Efficiency

An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli

In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either

CRISPR-based Gene Activation

Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.

MISSION shRNA Lentiviral Transduction

MISSION® shRNA Lentiviral Transduction application data

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

MISSION® Lentiviral Controls

When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative

Validating CRISPR/Cas9-mediated Gene Editing

After you have performed a CRISPR experiment it is important to determine which gRNAs performed successfully editing. There are many ways to validate CRISPR gene editing experiments. A quick and easy way to check for cutting is by using the

Long Oligos

Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.

shRNA Gene Families Sets

The MISSION® shRNA Library gene family sets are collections of genes related to specific cellular/molecular pathways documented in NCBI, Gene Ontology (GO), Protein Lounge, and Ingenuity Systems. Our Bioinformatics team has methodically mapped all TRC shRNA clones to each set.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.