Epigenetics

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DNA Methylation and Bisulfite Conversion

There are several common ways to determine whether a gene contains methylated DNA. Since mammalian methylation occurs at cytosines, researchers take advantage of the fact that methylated cytosine (meC) is stable to bisulfite treatment but unmethylated cytosine is transformed to

Guides to cell number for ChIP and endpoint analysis

Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis

Fragmentation of chromatin in ChIP

Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)

Agarose Beads Vs. Magnetic Beads in ChIP

Agarose beads Vs. Magnetic beads in Chromatin Immunoprecipitation (ChIP)

Global DNA Methylation Quantification

The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The

ChIP-X: Cross-linking proteins to DNA and cell lysis

X-ChIP: Cross-linking proteins to DNA and cell lysis

Chromatin Immunoprecipitation (ChIP) Troubleshooting

Get answers to frequently asked questions about chromatin immunoprecipitation (ChIP), including issues with sonication and DNA shearing.

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Introduction to DNA Methylation

Epigenetic regulation starts with DNA wound around a set of completely acetylated histones associated with an activated, fully transcribed gene.

Epigenetics Overview

Epigenetics is a term coined to describe changes that are not mutation based but can still be passed on from generation to generation. Genes that are activated or repressed without any change in DNA sequence are epigenetically controlled. Epigenetic modifications