Protein expression

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Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues, the primary and first pass site of metabolism occurs

Directed Evolution

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Melatonin Receptors

We offer many products related to melatonin receptors for your research needs.

Voltage Gated

Cell Signaling & Neuroscience Voltage-Gated Ion Channels. The majority of ion channels fall into two broad categories: voltage-gated ion channels (VGIC) and ligand-gated ion channels (LGIC). Members of the VGIC superfamily are usually closed at the resting potential of

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative

GABAB Receptors

We offer many products related to GABAB receptors for your research needs.

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere with the processes at the 30S subunit or 50S subunit of the 70S bacterial

Duolink® Proximity Ligation Assay Instructional Video

This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.

Cytochrome P450 Induction Assays

The cytochrome P450 (CYP450) induction assay provides a means to assess whether a test compound increases the production of specific CYP450 enzymes. We provide CYP450 induction assays for all small molecule formulations such as pharmaceuticals, industrial chemicals, and consumer products.

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

EX-CELL® Glycosylation Adjust (GAL+)

Our protein quality supplement, EX-CELL® Glycosylation Adjust (Gal+), provides customers with a novel chemically defined product which targets glycosylation attributes.

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Understanding Sweet Taste Perception

The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose.

Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

Detection of Protein-protein Interactions by in situ PLA

Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Metabolic Pathways

IUBMB-Sigma- Nicholson Metabolic Pathway Charts. The 22nd edition of the IUBMB-Sigma-Nicholson Metabolic Pathways Chart contains updated pathways involved in ATP metabolism in the mitochondria and chloroplast. It contains the Glycolytic Pathway followed by the TCA (Krebs) Cycle and the Respiratory

Glycolysis via the Embden-Meyerhof-Parnas Glycolytic Pathway

Review the 10 steps of glycolysis in the Embden-Meyerhof-Parnas glycolytic pathway. Easily compare reaction stages and buy the enzymes for your life science research.

How Proximity Ligation Assays (PLA) Work

Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

Competent Cell Selection & E. coli Markers Guide

Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.

Epidermal Growth Factor Receptor (EGFR) Signaling

Review EGF/EGFR signaling (epidermal growth factor receptor) and find links to related products.

Protein-based Drug Transport

Protein-based drug transporters are expressed in Sf9 cells. Understanding the specific mechanisms of tumor cell transporters is an essential aspect of chemotherapeutic drug design.

AMPKs

The AMP-activated protein kinase (AMPK) acts as a sensor of cellular energy status. AMPK exists as heterotrimeric complexes comprising a catalytic α subunit and regulatory β and γ subunits.

Nitric Oxide Synthases

We offer many products related to nitric oxide synthases for your research needs.

Mitogen-activated Protein Kinases (MAPKs)

The mitogen-activated protein kinase (MAPK) family consists of stress-activated (SAPK) and MAPKs. Learn about their network of signal transduction cascades that mediate cellular responses to a diverse range of stimuli.

Glycogen Synthase Kinase 3 (GSK-3) Overview

Glycogen synthase kinase 3 (GSK-3) is a highly conserved family of serine/threonine kinases for over 100 proteins in many pathways. See a list of accepted GSK-3 modulators and related products.

Phosphoprotein Phosphatases (Serine/Threonine)

We offers many products related to phosphoprotein phosphatases (Serine/Threonine) for your research needs.

Galanin Receptors

We offer many products related to galanin receptors for your research needs.

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