pcr cyclers

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What is Hot Start PCR?

Learn about the benefits of using Hot Start PCR reagents to suppress enzymatic activity before PCR reaction tubes enter the thermal cycler.

Troubleshooting PCR and RT-PCR Amplification

This page shows PCR and RT-PCR amplification troubleshooting.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Multiplex Real-Time PCR

Multiplex qPCR employing probe-based chemistries is a demanding application that often requires extensive optimization and validation.

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Expand™ Long Template PCR System Protocol Troubleshooting

This polymerase mixture produces a high yield of PCR product from genomic DNA.

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Real-Time PCR with IMS Technology

Rapid PCR detection with Assurance GDS system using IMS technology offers sensitive, specific, and reliable food pathogen detection.

Extract-N-Amp™ FFPE Tissue PCR Protocol

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.

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