- Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.
Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.
Antimicrobial agents and chemotherapy (2007-09-19)
Fátima Fonseca, Ana Cristina Sarmento, Isabel Henriques, Bart Samyn, Jozef van Beeumen, Pedro Domingues, Maria Rosário Domingues, Maria José Saavedra, António Correia
PMID17875998
ABSTRACT
The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, pH 8.0, ~0.5 M in H2O