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Microbial Mutagenicity Assay: Ames Test.

Bio-protocol (2018-03-20)
Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala, Pradeep Bhatnagar
ABSTRACT

The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, reagents, cosmetics, waste water, pesticides and other substances which are easily solubilized in a liquid suspension. We present the protocol for conducting Ames test in the laboratory.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Nitroquinoline N-oxide, ≥98%
Sigma-Aldrich
Crystal Violet, ACS reagent, ≥90.0% anhydrous basis
Sigma-Aldrich
Benzo[a]pyrene, ≥96% (HPLC)