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  • [Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].

[Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].

Guang pu xue yu guang pu fen xi = Guang pu (2003-08-14)
Hai-yan Xiang, Xiao-ming Chen, Di-wu Zhou
ABSTRACT

The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Basic Fuchsin, Dye content >85 %
Sigma-Aldrich
Pararosaniline acetate, Dye content 90 %
Sigma-Aldrich
Pararosaniline hydrochloride, >85.0% (HPLC)
Sigma-Aldrich
Basic Fuchsin, certified by the BSC, Dye content ≥88 %