- Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.
Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.
The Biochemical journal (1990-03-15)
D R Shackleton, D J Hulmes
PMID1970238
ABSTRACT
A method is described for the purification of piglet skin lysyl oxidase from a crude urea extract by two simple chromatographic procedures. The method relies on the selective interaction between Sephacryl S-200 and lysyl oxidase that occurs in the absence of urea and in conditions of low ionic strength.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Sephacryl®, 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)
Sigma-Aldrich
Sephacryl®, 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)