The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

Experientia (1982-04-15)

H Robern

ABSTRACT

A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.

MATERIALS

Product Number

Brand

Product Description

S500HR

Sigma-Aldrich

Sephacryl^®, 500-HR, MW range 40-20,000 kDa (dextrans)

S400HR

Sigma-Aldrich

Sephacryl^®, 400-HR, MW range 20-8000 kDa (globular proteins)

S100HR

Sigma-Aldrich

Sephacryl^®, 100-HR, MW range 1000-100,000 Da (globular proteins)

S300HR

Sigma-Aldrich

Sephacryl^®, 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

S200HR

Sigma-Aldrich

Sephacryl^®, 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)