[Characteristics of toxigenic properties of Myrothecium cinctum (Corda) sacc., 1886 and Myrothecium commune pidopl., 1969].

[Characteristics of toxigenic properties of Myrothecium cinctum (Corda) sacc., 1886 and Myrothecium commune pidopl., 1969].

Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) (2014-01-24)

A M Zaĭchenko, E S Tsyganenko, E V Andrienko, Ia I Savchuk

ABSTRACT

The comparative study of toxigenic potential of Myrothecium cinctum (12 strains) and Myrothecium commune (7 strains) was conducted. The ability to produce the complex of macrocyclic trichothecenes (MCTC) presented in ethanol soluble fraction (ESF), as well as fungistatic activity against yeasts test-cultures (strains Saccharomyces vini "Feodosia" and Candida stellatoidea 62) and rabbit skin test were evaluated. It was established that ESF output varied within wide limits in all studied strains of both species but it was significantly lower in M. commune strains. Higher ESF yields were accompanied as a rule by higher fungistatic activity and skin reaction in rabbits. The separate strains showed high biological activity with minor ESF output that could be possible caused by domination of more toxic components in complex preparation. The study of MCTC component structure by high performance liquid chromatography method (HPLC) showed with a few exceptions the presence of verrucarins A and J, roridins A, E, and H as well as unidentified components whose column retention times were 2.1 and 4.9 minutes. The production of roridin H and unknown component with 2.1 min. retention time was typical of M. cinctum strains while verrucarin A and unidentified component with 4.9 min. retention time was typical of M. commune strains.

MATERIALS

Product Number

Brand

Product Description

PHR1070

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

277649

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2858

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676829

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362808

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E-053

Supelco

Ethanol-500, 500 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-056

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Ethanol-20 (10 ampules/kit), 20 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-034

Supelco

Ethanol Calibration Kit, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-035

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Ethanol-25, 25 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-036

Supelco

Ethanol-400 (10 ampules/kit), 400 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

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Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

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270741

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29221

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Supelco

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Ethyl alcohol, Pure, 190 proof, meets USP testing specifications

493511

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51976

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Ethanol, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)

E-031

Supelco

Ethanol-100 (10 ampules/kit), 100 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-040

Supelco

Ethanol-10, 10 mg/dL in H₂O, pack of 10 × 1.2 mL ampules, certified reference material, Cerilliant^®

E-041

Supelco

Ethanol-150, 150 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-032

Supelco

Ethanol-200 (10 ampules/kit), 200 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-033

Supelco

Ethanol-300, 300 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-045

Supelco

Ethanol-40, 40 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-030

Supelco

Ethanol-80, 80 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

E-029

Supelco

Ethanol-50, 50 mg/dL in H₂O, ampule of 10 × 1.2 mL, certified reference material, Cerilliant^®

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Sigma-Aldrich

Ethanol, puriss. p.a., absolute, ≥99.8% (GC)